Hydroxysafflor yellow A protects rat brains against ischemia-reperfusion injury by antioxidant action

doi:10.1016/j.neulet.2005.05.069

Neuroscience Letters

Volume 386, Issue 1, 23 September 2005, Pages 58-62

https://doi.org/10.1016/j.neulet.2005.05.069 Get rights and content

Abstract

The present study was conducted to investigate whether hydroxysafflor yellow A (HSYA) has a protective effect on brain injury after focal cerebral ischemia reperfusion, and to determine the possible mechanism. Behavioral tests were used to evaluate the damage to central nervous system. The infarct volume of brain was assessed in brain slices stained with 2% solution of 2,3,5-triphenyl tetrazolium chloride (TTC). Adult male Wistar rats were subjected to 2 h of middle cerebral artery occlusion and 24 h of reperfusion. Spectrophotometric assay was used to determine the content of malondialdehyde (MDA), and the activity of total antioxidative capability (T-AOC) and superoxide dismutase (SOD). The results showed that treatment with HSYA (2, 4, 8 mg/kg, i.v.) significantly decreased neurological deficit scores and reduced the percentage of infarction in the ipsilateral hemisphere compared with the model group. At the same time, HSYA treatment significantly attenuated the elevation of MDA content, the decrease in SOD activity, and the T-AOC in the ipsilateral hemisphere and serum. All of these findings suggest that HSYA might provide neuroprotection against cerebral ischemia/reperfusion injury through its antioxidant action.

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Acknowledgment

This study was supported by School of Medicine, Shandong University and the Nature Science Foundation of Shandong Province, No. Q2004C05.

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Neuroscience Letters

Abstract

Section snippets

Acknowledgment

References (16)

Brain ischemia and reperfusion: molecular mechanisms of neuronal injury

J. Neurol. Sci.

Nimodipine attenuates biochemical, behavioral and histopathological alterations induced by acute transient and long-term bilateral common carotid occlusion in rats

Pharmacol. Res.

Separation and identification of safflower yellow A and carthamin from Carthamus tinctorius L

Chin. Tradit. Herb. Drugs.

Alterations in superoxide dismutase, glutathione peroxidase and catalase activities in experimental cerebral ischemia-reperfusion

Res. Exp. Med. (Berl.)

Antioxidative effect of hydroxysafflor yellow A

Chin. Tradit. Herb. Drugs.

Hypotensive effects of safflower yellow in spontaneously hypertensive rats and influence on plasma renin activity and angiotensin II level

Acta Pharm. Sin.

Oxidative stress in brain ischemia

Brain Pathol.

New models of focal cerebral ischemia

Br. J. Clin. Pharmacol.

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Safflomin A: A novel chemical marker for Carthamus tinctorius L. (Safflower) monofloral honey

Protective effect of Hydroxysafﬂor Yellow A on cerebral ischemia reperfusion-injury by regulating GSK3β-mediated pathways

Biphasic Fermentation of Trapa bispinosa Shells by Ganoderma sinense and Characterization of Its Polysaccharides and Alcoholic Extract and Analysis of Their Bioactivity

The Extract of Astragalus membranaceus Inhibits Lipid Oxidation in Fish Feed and Enhances Growth Performance and Antioxidant Capacity in Jian Carp (Cyprinus carpio var. Jian)

OTUD1 ameliorates cerebral ischemic injury through inhibiting inflammation by disrupting K63-linked deubiquitination of RIP2