Whole genome sequencing (WGS) paved the way to sequence the mitochondrial genomes with greater ease.
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WGS data of C. batrachus, generated from 2 sequencing platforms, were utilized for complete mitogenome sequence retrieval.
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De novo and reference based assembly yielded similar results with best assembly of 16,510 bp size (GenBank Acc. KM259918).
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The mitogenome of annotation of C. batrachus resulted in 13 coding genes, 22 tRNA genes and 2 rRNA genes.
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The phylogenetic analysis of coding DNA sequences (CDS) and tRNA supports the monophyly of catfishes.
Abstract
Whole genome sequencing (WGS) using next generation sequencing technologies paves the way to sequence the mitochondrial genomes with greater ease and lesser time. Here, we used the WGS data of Clarias batrachus, generated from Roche 454 and Ion Torrent sequencing platforms, to assemble the complete mitogenome using both de novo and reference based approaches. Both the methods yielded almost similar results and the best assembled mitogenome was of 16,510 bp size (GenBank Acc. No. KM259918). The mitogenome annotation resulted in 13 coding genes, 22 tRNA genes, 2 rRNA genes and one control region, and the gene order was found to be identical with other catfishes. Variation analyses between assembled and the reference (GenBank Acc. No. NC_023923) mitogenome revealed 51 variations. The phylogenetic analysis of coding DNA sequences and tRNA supports the monophyly of catfishes. Two SSRs were identified in C. batrachus mitogenome, out of which one was unique to this species. Based on the relative rate of gene evolution, protein coding mitochondrial genes were found to evolve at a much faster pace than the d-loop, which in turn are followed by the rRNAs; the tRNAs showed wide variability in the rate of sequence evolution, and on average evolve the slowest. Among the coding genes, ND2 evolves most rapidly. The variations present in the coding regions of the mitogenome and their comparative analyses with other catfish species may be useful in species conservation and management programs.
