Original articleEffects of dichloroacetate and ubiquinone infusions on glycolysis activity and thermal sensitivity during sepsis
Section snippets
Animals
Fifteen male Sprague-Dawley rats weighing 400 to 500 g were used in the study. On their arrival at the laboratory, they were kept in a special room and supplied with water and food ad libitum for at least 3 days before being subjected to any handling. After the induction of anesthesia with the use ofintraperitoneal ketamine (11 mg/100 g) and xylazine (0.5 mg/100 g), we laid the animals out on a thermoregulated mattress to keep their core temperatures constant during the control phase. We
Glucose and lactate measurements
We observed increased plasma lactate levels and a significant increase in muscle glucose content at 4 hours for all animals in the control group (P < .05; Table I). No lactatemia variation was noted, but we saw a decrease in muscle lactate content (P < .05) and an increase in muscle glucose content (P < .05) in the animals assigned to the DCA group. We noted only increased plasma lactate levels in the animals in the CoQ10 group.
Glycolysis activity
At a 37°C measurement temperature, we noted an overall increase in
Discussion
As observed in previous studies, animals demonstrate significant increases in glycolytic flux within 4 hours of the induction of sepsis induction3, 7; this effect is less pronounced in the hyperthermia range than in the hypothermia range (Table III). The absence of glycolytic flux increase in response to a temperature increase in septic control animals within the hyperthermia range (decreased Q10) as depicted in this experiment, associated with decreased oxidative phosphorylation as already
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