Regenerative EndodonticsEvaluation of the Biodistribution of Human Dental Pulp Stem Cells Transplanted into Mice
Section snippets
Isolation, Culture, and Characterization of hDPSCs
Volunteers were recruited from patients who were scheduled for third molar extraction at Yonsei University Dental Hospital, Seoul, South Korea. Informed consent was obtained from each volunteer, and the research protocol was approved by the Institutional Review Board of Yonsei University Dental Hospital (Institutional Review Board number: 2-2015-0055). Normal impacted third molars were collected from adults (19–29 years of age). After extraction, the pulp tissue was gently separated from the
Biodistribution of hDPSCs in Mice
hDPSCs were identified on the basis of stem cell markers and differentiation potentials. Flow cytometry analysis revealed that the cells were positive for CD73 (98.4%), CD90 (99.3%), and CD105 (98%) and negative for CD45 (0.1%). Compared with bone marrow MSCs, the osteogenic, adipogenic, and chondrogenic differentiation potential of hDPSCs was verified (Fig. 2A–J).
For the intravenous injection group, all animals (n = 28) survived after hDPSC transplantation. The time course detection of the
Discussion
In this study, in vivo biodistribution of intravenously injected and intrapulpally transplanted hDPSCs was compared. The hDPSCs used in this study were isolated and cultured according to the method described by Gronthos et al (2). In order to determine whether these cells met the criteria for MSCs, as provided by the International Society for Cellular Therapy in 2006 (19), we characterized the cells with respect to the surface marker expression and multilineage differentiation potential. Our
Acknowledgments
Sunil Kim and Sukjoon Lee contributed equally to this work.
Supported by the Korean Health Technology R&D Project, Ministry of Health and Welfare, Republic of Korea (grant no. HI14C1817); the Yonsei University College of Dentistry (grant no. 6-2016-0025), and in part by the American Association of Endodontists Foundation.
The authors deny any conflicts of interest related to this study.
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