Basic ResearchEvaluation of Residual Antibacterial Effect of Human Radicular Dentin Treated with Triple and Double Antibiotic Pastes
Section snippets
Bacterial Strain and Media
Anaerobic blood agar plates (CDC; BioMerieux, Durham, NC) were used to initially grow and maintain Enterococcus faecalis bacteria (American Type Culture Collection 29212). Brain-heart infusion broth supplemented with 5 g/L yeast extract was used to grow the bacterium at 37°C in an anaerobic environment using gas-generating sachets (GasPak EZ; Becton, Dickinson and Company, Franklin Lakes, NJ) to produce the required environment.
Antibiotic Paste Preparation
For TAP, 1000 mg, 1 mg, or 0.5 mg United States Pharmacopeia grade
Results
The mixed linear model indicated significant effects of treatment type, post-treatment time in PBS, and their interaction on the residual antibacterial properties of radicular dentin (P < .05). All dentin specimens treated with antibiotics showed a gradual reduction in residual antibacterial effect over time (P < .05). Figure 1 shows that dentin treated with 1000 mg/mL TAP demonstrated a significant residual antibacterial effect compared with the untreated control group in up to 14 days of
Discussion
The residual antibiotic effect represents the capability of the antibiotic medicament to bind to the tooth structure and the subsequent release in an active form (17). A previous study suggested that TAP has the ability to bind to dentin structure (14). However, no previous research has measured the antibacterial effect of the retained medicaments. The lack of canal filling during the regeneration process may facilitate the repopulation of residual bacteria inside the canal space (18).
Acknowledgments
The authors deny any conflicts of interest related to this study.
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