Basic research—technologyIn Vitro Evaluation of the Sealing Ability of Newly Developed Calcium Phosphate–based Root Canal Sealer
Section snippets
Preparation of Samples
We used 115 freshly extracted human teeth with single, straight canals. This study was carried out with the Seoul National University Dental Hospital IRB approval. All teeth were sectioned at the cemento-enamel junction, and the coronal portion of the roots was removed. The working length for each root was established at 1 mm short of the apical foramen with a #15 file. Each canal was instrumented to a size #40 master apical file by the modified crown-down technique with a Profile (Maillefer,
Results
All specimens of the positive control group showed broth turbidity and a color change in the lower chamber within 2 days after inoculation. By contrast, there was no change in the negative control group throughout the experiment.
Table 1 shows the number of teeth and the total percentage leakage of P. nigrescens through the root canal filling after 30, 60, and 90 days. There was a statistically significant difference between SARCS (I & III) and the other sealers including CAPSEAL (P < .05) after
Discussion
Using bacteria as a leakage tracer would provide more biologically significant and clinically relevant information (4). Bae et al (5) found Fusobacterium nucleatum produced turbidity and a color change in this model in 1 day and remained viable for 2 weeks. In fact, 73.2% of black-pigmented bacteria isolated from endodontic infections, which were previously identified as P. intermedia, should be classified as P. nigrescens (6), and P. nigrescens was reported the most often isolated
Acknowledgments
This study was supported by a grant of the Korea Health 21 R&D Project, Ministry of Health & Welfare, Republic of Korea (01-PJ5-PG1-01CH12-0002).
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