Research paperEnhancement of antibody fragment secretion into the Escherichia coli periplasm by co-expression with the peptidyl prolyl isomerase, FkpA, in the cytoplasm
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Abbreviations
Fab
fragment-antigen-binding
Fc
fragment crystallizable
Fd
fragment of antibody consisting of CH1 and VH
ScFv
single-chain variable fragment
VH
variable heavy
VL
variable light
PPIase
peptidyl prolyl cis-trans isomerase
PCR
polymerase chain reaction
IPTG
isopropyl β-d-1-thiogalactopyranoside
OD
optical density
PBS
phosphate buffer saline
M.O.I.
multiplicity of infection
PEG
polyethylene glycol
HRP
horseradish peroxidase
ELISA
enzyme-linked immunosorbent assay
TMB
3,3′,5,5′-tetramethylbenzidine
huINSR
human insulin receptor
BSA
bovine serum albumin
kd
dissociation constant
RT
room temperature
EC50
half maximal effective concentration
CFU
colony-forming units
SD
Shine–Dalgarno sequence
PVDF
polyvinylidene fluoride
SPR
Surface Plasmon Resonance
SDS
sodium dodecyl sulfate
SEM
Standard Error of the Mean
Keywords
Chaperones
Bacteria
Cytoplasm
Phage display
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Copyright © 2013 The Authors. Published by Elsevier B.V.