Effect of Ecklonia cava polyphenol on adiposity reduction is associated with gut microbiota composition in subjects with abdominal obesity: A secondary analysis

https://doi.org/10.1016/j.jff.2022.105333Get rights and content
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Highlights

  • Ecklonia cava polyphenol (EP) reduced adiposity in abdominal obese adults.

  • EP changed gut Firmicutes-to-Bacteroidetes ratio and microbiota composition.

  • EP-induced microbiota alterations were associated with improved adiposity.

  • Predicted KEGG pathway revealed metabolic functional alterations by EP.

Abstract

A previous randomized controlled trial showed that Ecklonia cava polyphenol (EP) has the potential to reduce fat accumulation based on clinical outcomes and target gene expression analysis. Here, we investigated whether EP affects gut microbiota composition and impacts clinical outcomes. To this end, we analyzed 16S rRNA sequencing on fecal samples from subgroup subjects with abdominal obesity (n = 20/group). Compared with the placebo treatment, EP supplementation attenuated the increase of the Firmicutes-to-Bacteroidetes ratio and changed 18 genera composition, including genera related to obesity and fermentation of unabsorbed compounds. Furthermore, Spearman's correlation and Tax4Fun analysis demonstrated that alterations in genera are tightly associated with reducing adiposity via regulating oxidative stress. Together, these findings suggest that the gut microbiota-regulating effect might be a possible mechanism of EP-induced adiposity reduction in subjects with abdominal obesity.

Keywords

Association
Human clinical trial
Gut microbiota
Obesity
Phlorotannin
Functional profile prediction

Abbreviations

ANCOM
analysis of the composition of microbiomes
BMI
body mass index
CAT
catalase
CLR Perm
centered log-ratio transformation and permutation logistic regression model
CONSORT
Consolidated Standards of Reporting Trials
DBP
diastolic blood pressure
F:B ratio
Firmicutes-to-Bacteroidetes ratio
FMI
fat mass index
GPx
glutathione peroxidase
HPLC
high-performance liquid chromatography
KEGG
Kyoto Encyclopedia of Genes and Genomes
KO
Kyoto Encyclopedia of Genes and Genomes Orthologus
ldl-cholesterol
low density lipoprotein cholesterol
HDL-cholesterol
high density lipoprotein cholesterol
MDA
malondialdehyde
OTU
operational taxonomic unit
Ox-LDL
oxidized-low-density lipoprotein
RFS
recommended food score
SBP
systolic blood pressure
SCFA
short-chain fatty acid
EP
Ecklonia cava polyphenol
SMI
skeletal muscle index
SOD
superoxide dismutase
ZIBSeq
zero-inflated beta regression
ZIG
zero-inflated Gaussian mixture model

Data availability

Mendeley data: SeaPN_BM (DOI: 10.17632/cfwj5hxb3r.1)

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1

These authors contributed equally to this work.