Airway epithelial cell–pathogen interactions

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Abstract

Cystic fibrosis (CF) airway becomes colonized with only a limited number of bacterial pathogens. It is of paramount importance to establish in vitro and in vivo models to better understand bacterial–host interactions under CF-like conditions. In this article, in vitro methods suitable to study Pseudomonas aeruginosa (Pa) and Staphylococcus aureus (Sa) adherence to and uptake by airway epithelial cells are described. Acute and chronic respiratory infection models, which have been used in CF transgenic mice and mimic human CF lung pathology, are also taken into consideration.

Keywords

Airway epithelial cells
Infection
Mouse models
Pseudomonas aeruginosa
Staphylococcus aureus

Abbreviations

Ab
antibody
ALI
air–liquid interface
Bc
Burkholderia cepacia
cfu
colony-forming units
CK
cytokeratin
DAR
donkey antirabbit
ELISA
enzyme-linked immunosorbent assay
EM
electron microscopy
FITC
fluorescein isothiocyanate
Hi
Haemophilus influenzae
IgG
G immunoglobulins
LPS
(bacterial) lipopolysaccharides
Pa
Pseudomonas aeruginosa
PBS
phosphate-buffered saline
Sa
Staphylococcus aureus
SEM
scanning electron microscopy
Sm
Stenotrophomonas maltophilia
TEM
transmission electron microscopy
TSA
tryptic soy agar
3-D
three-dimmensional

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