De novo transcriptome analysis of Pleurotus djamor to identify genes encoding CAZymes related to the decomposition of corn stalk lignocellulose

https://doi.org/10.1016/j.jbiosc.2019.04.011Get rights and content

CAZymes play a very important role in the biotransformation of corn stalk biomass, which is an important resource for sustainable development. Pleurotus djamor can produce CAZymes related to the decomposition of corn stalk lignocellulose biomass in sole corn stalk substrate; however, little is known about their encoding genes. In order to identify CAZymes encoding genes, RNA high-throughput sequencing of P. djamor was performed in this study. The results showed that a core set of 70 upregulated genes encoding putative CAZymes were revealed. They encode 19 kinds of CAZymes in total, of which there are 4 EGLs, 8 CBHs, 5 BGLs, and 12 LPMOs related to cellulose degradation, 8 XYNs, 1 XYL, 2 AGUs, 3 ABFs, 2 AGLs, and 2 AXEs related to hemicellulose degradation, and 5 LACCs, 2 MnPs, 5 VPs, 3 CDHs, 1 AAO, 1 GOX, 1 AOX, 2 GAOXs, and 3 GLOXs related to lignin degradation. This variety suggests that CAZymes may play a very important role in decomposing the lignocellulose biomass of corn stalk. This is the first study to report the de novo transcriptome sequencing of P. djamor, which will produce a dataset of genes encoding CAZymes, thereby laying the foundation to elucidate the degradation mechanism of corn stalk biomass and boost the biotransformation of corn stalk biomass resources.

Section snippets

Fungal strain and culture conditions

P. djamor CM13 was stored on Potato Dextrose Agar (PDA) plates at 4 °C. For pre-culturing, the strain was incubated at 28 °C for 6–7 days on PDA plates. Three mycelia-covered agar plugs (diameter = 1 mm) from the PDA plates were inoculated into PDA liquid medium (PDL), and then incubated at 28 °C with shaking at 150 rpm for eight days. Then, 10 mL of the mycelia suspension from the PDL was transferred into 90 mL of corn stalk liquid medium (CSL, 30 g/L corn stalk powder with a particle size of

Lignocellulose-degrading enzymes activities of P. djamor

Cellulase and xylanase activities of P. djamor growing in CSL increased with the prolongation of the incubation time, reaching the highest activity on the 16th day, and then decreasing (Fig. 1A). Laccase and MnP activities reached their peak values on the 4th day and then decreased rapidly; however, they were still maintained at low levels (Fig. 1B). Considering that the apoenzyme expression occurs after RNA transcription and the action of cellulase and xylanase, the mycelia cultured in CSL for

Acknowledgments

This work was supported by Science and Technology Department of Jilin Province, China [20180101248JC] and Education Department of Jilin Province, China [JJKH20180681KJ].

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