De novo transcriptome analysis of Pleurotus djamor to identify genes encoding CAZymes related to the decomposition of corn stalk lignocellulose
Section snippets
Fungal strain and culture conditions
P. djamor CM13 was stored on Potato Dextrose Agar (PDA) plates at 4 °C. For pre-culturing, the strain was incubated at 28 °C for 6–7 days on PDA plates. Three mycelia-covered agar plugs (diameter = 1 mm) from the PDA plates were inoculated into PDA liquid medium (PDL), and then incubated at 28 °C with shaking at 150 rpm for eight days. Then, 10 mL of the mycelia suspension from the PDL was transferred into 90 mL of corn stalk liquid medium (CSL, 30 g/L corn stalk powder with a particle size of
Lignocellulose-degrading enzymes activities of P. djamor
Cellulase and xylanase activities of P. djamor growing in CSL increased with the prolongation of the incubation time, reaching the highest activity on the 16th day, and then decreasing (Fig. 1A). Laccase and MnP activities reached their peak values on the 4th day and then decreased rapidly; however, they were still maintained at low levels (Fig. 1B). Considering that the apoenzyme expression occurs after RNA transcription and the action of cellulase and xylanase, the mycelia cultured in CSL for
Acknowledgments
This work was supported by Science and Technology Department of Jilin Province, China [20180101248JC] and Education Department of Jilin Province, China [JJKH20180681KJ].
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