Enhanced biohydrogen production from corn stover by the combination of Clostridium cellulolyticum and hydrogen fermentation bacteria
Section snippets
Materials and bacterial strains
Corn stover was harvested from a rural area near Beijing and pretreated by steam explosion as previously described (26).
The hydrogen-producing strains of E. aerogenes IAM1183 and C. paraputrificum M-21 were previously described by Lu et al. (9) The E. cloacae (NBRC 12935) and C. amalonaticus Y19 (NBRC 13547) strains were purchased from NBRC, and the cellulolytic strain of C. cellulolyticum (DSM 5812) was purchased from DSMZ.
Medium and cultivation
The preculture medium (1000 mL) for C. cellulolyticum consisted of
Hydrogen production from corn stover by co-culture fermentation
The strains used in this study include cellulolytic and non-cellulolytic mesophilic strains (Table 2). C. cellulolyticum was used as the cellulolytic bacterium. C. paraputrificum, C. amalonaticus, E. cloacae and E. aerogenes were used as the hydrogen fermentation bacteria. The optimum temperatures and pH ranges of the different strains were similar. The cellulolytic and non-cellulolytic hydrogen-evolving bacteria were paired to compare their hydrogen production efficiencies with 25 g/L
Acknowledgments
This work was supported by the National Key Technology R and D Program of China (2014BAD02B03) and the NSFC-JST Collaborating Research Project (21161140328).
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