Kinetics of bisphenol A degradation by Sphingomonas paucimobilis FJ-4
Section snippets
Bacterial strain
A BPA-degrading bacterium S. paucimobilis FJ-4 was used for this study. This strain was isolated from activated sludge treating wastewater of an epoxy resin manufacturing plant. This bacterium can grow on BPA up to 228 mg/L as the sole carbon and energy sources. Optimal pH and temperature for BPA degradation in this bacterium are about 7.0 and 30°C, respectively 2, 12.
Culture media
The basal salt medium (BSM) used for this study contained 1.0 g of K2HPO4, 1.0 g of (NH4) 2SO4, 0.2 g of MgSO4·7H2O, 0.01 g of
Results
S. paucimobilis FJ-4 bacteria were cultivated in the chemostat to investigate its kinetic parameters on BPA degradation. Results of experimental runs conducted at different cell retention times are shown in Fig. 1. Each experimental run was conducted at least three times longer than the corresponding cell retention time. At the short cell retention time of 8 h, the bacterial cells were washed out from the chemostat. At cell retention times longer than 12 h, steady-states of the bacterial growth
Discussion
This report is the first of a kinetic parameter set (kmax, Ks, Y, and b) of bacteria grown on BPA in a pure culture chemostat. Typical pathways of bacterial BPA degradation are oxidative skeletal rearrangement, ipso-substitution, and meta-cleavage after aromatic ring hydroxylation 7, 13. Among those pathways, the oxidative skeletal rearrangement is used by many BPA-utilizing bacteria, such as strain MV-1 14, 15, Sphingomonas bisphenolicum AO1 (16), Sphingomonas sp. BP-7 (17), and S. paucimobilis
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