Antioxidant and antityrosinase proanthocyanidins from Polyalthia longifolia leaves
Section snippets
Plant materials
The leaves of P. longifolia were collected from the campus of Xiamen University (Xiamen, China). The leaves were freeze dried and then ground using a cutting mill (model BL301D5; Saikang, China) to obtain fine powder. The powder was stored at −20°C in a freezer prior to analysis.
Chemicals
Sephadex LH-20, amberlite IRP-64 cation-exchange resin, benzyl mercaptan, cesium chloride, catechin, epicatechin, l-3,4-dihydroxyphenylalanine (l-DOPA), ascorbic acid, 2,4,6-tripyridyl-S-triazine (TPTZ),
Results and discussion
Due to the complexity and diversity nature of PAs, the task of characterizing their structures remains very challenging. Various techniques including NMR, HPLC-ESI-MS, and MALDI-TOF MS have been used to characterize their structures (15).
In the present research, the liquid state 13C NMR spectrum of the PAs from leaves of P. longifolia was analyzed and the result given in Fig. 2. The signal assignment was with reference to the report of Chai et al. (10). The 13C NMR spectrum in DMSO-d6 showed
Acknowledgments
The present investigation was supported by the National Natural Science Foundation of China (nos. 31371857 and 31371988), the National Science Foundation for Fostering Talents in Basic Research of the National Natural Science Foundation of China (Grant no. J1310027/J0106), and the the Natural Science Foundation of Fujian Province, China (no. 2012J01151).
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2022, LWTCitation Excerpt :The efficient inhibition may largely depend on the structural features of condensed tannins (Monagas, Quintanilla-Lopez, Gómez-Cordoves, Bartolome, & Lebron-Aguilar, 2010). According to the reports related to some other condensed tannins, the IC50 values of inhibition of diphenolase activity by condensed tannins from P. longifolia leaf (Chen et al., 2014) and L. leucocephala leaf (Chen et al., 2018) were 773.09 ± 1.47 and 27.2 ± 1.4 μg/mL, respectively. Evidently, ECTs exhibited higher inhibition potential on diphenolase than P. longifolia leaf condensed tannins.
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The first two authors contributed equally to this work.