Mechanisms of asthma and allergic inflammationSurface CD88 functionally distinguishes the MCTC from the MCT type of human lung mast cell
Section snippets
Culture of Lu-MC and Sk-MC
All experimental protocols involving human tissues were approved by the Human Studies Committee at Virginia Commonwealth University. Surgical lung or skin tissue samples were obtained from consented patients through the Cooperative Human Tissue Network (Columbus, Ohio), the National Disease Research Interchange Center (Philadelphia, Pa), or the Departments of Surgery or Pathology at Virginia Commonwealth University. Lung specimens were typically lobectomies from patients with lung cancer,
CD88-dependent sorting of MCT and MCTC cells from lung
Because MCTC cells from skin express the C5aR, CD88, experiments were conducted to see whether CD88 also was expressed on MCTC cells from lung and could enable MCTC cells to be separated from MCT cells by cell sorting. As shown in Fig 1, after sorting Lu-MC, <4% of the cells in the Kit+/CD88− gate were chymase+, whereas greater than 98% of the cells in the Kit+/CD88+ gate were chymase+. This result was representative of 3 independent experiments. By real-time/RT-PCR, the 18S-rRNA–normalized
Discussion
The current study makes 3 important observations. First, the mature MCTC type of mast cell in lung and skin expresses CD88, the receptor for C5a, whereas mature MCT cells in lung do not. Accordingly, cell sorting of Kithi lung-derived mast cells with anti-CD88 antibody separates CD88− MCT from CD88+ MCTC cells. Thus, surface expression of CD88 corresponds to the MCTC phenotype, regardless whether the MCTC cell originates from the skin or lung. Lu-MCT cells express negligible levels, if any, of
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Supported by grants R01-AI27517 and R01-AI20487 to Dr. Schwartz and K08-AI057357 to Dr Zhao from the National Institutes of Health.
Disclosure of potential conflict of interest: C. A. Oskertizian: none disclosed; W. Zhao: none disclosed; H.-K. Min: none disclosed; H.-Z. Xia: none disclosed; A. Pozez: none disclosed; J. Kiev: none disclosed; L. B. Schwartz: none disclosed.