J Appl Biomed 15:256-264, 2017 | DOI: 10.1016/j.jab.2017.06.001

RGD peptide doped polypyrrole film as a biomimetic electrode coating for impedimetric sensing of cell proliferation and cytotoxicity

Yuan Lia,b, Chao Yub,*
a Chongqing Medical University, Yongchuan Hospital, Chongqing, China
b Chongqing Medical University, Institute of Life Science, Chongqing, China

A novel and facile in vitro cell sensing system has been developed with one-step electropolymerization of the conducting polypyrrole(PPy) polymer using RGD peptide as the sole dopant on an indium tin oxide (ITO) surface. The resulted RGD peptide-doped polypyrrole (PPy/RGD) composite film had a robust surface, in which PPy provided a biocompatible matrix for cell growth and a conducting interface for electrical detection, while the RGD peptide entrapped in the PPy matrix conferred the desired biomimetic properties. Using the human lung cancer cell A549 as a model, this system can be used to monitor cell behaviors of proliferation and cytotoxicity.

Keywords: RGD peptide; Polypyrrole; Cell-based sensor; Proliferation; Cytotoxicity; Electrochemical impedance microscopy

Received: August 13, 2016; Revised: March 24, 2017; Accepted: June 19, 2017; Published: November 1, 2017  Show citation

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Li Y, Yu C. RGD peptide doped polypyrrole film as a biomimetic electrode coating for impedimetric sensing of cell proliferation and cytotoxicity. J Appl Biomed. 2017;15(4):256-264. doi: 10.1016/j.jab.2017.06.001.
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