iScience
Volume 25, Issue 7, 15 July 2022, 104626
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Article
Biogenic action of Lactobacillus plantarum SBT2227 promotes sleep in Drosophila melanogaster

https://doi.org/10.1016/j.isci.2022.104626Get rights and content
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open access

Highlights

  • Lactobacillus plantarum SBT2227 promotes sleep at the onset of nighttime

  • Existing intestinal microbes do not affect the SBT2227 sleep effect

  • Heat-stable intracellular/intramembrane components are candidates for active substances

  • Neuropeptide F is required for the sleep-promoting effect of SBT2227

Summary

Lactic acid bacteria (LAB) influence multiple aspects of host brain function via the production of active metabolites in the gut, which is known as the pre/probiotic action. However, little is known about the biogenic effects of LAB on host brain function. Here, we reported that the Lactobacillus plantarum SBT2227 promoted sleep in Drosophila melanogaster. Administration of SBT2227 primarily increased the amount of sleep and decreased sleep latency at the beginning of night-time. The sleep-promoting effects of SBT2227 were independent of the existing gut flora. Furthermore, heat treatment or mechanical crushing of SBT2227 did not suppress the sleep-promoting effects, indicative of biogenic action. Transcriptome analysis and RNAi mini-screening for gut-derived peptide hormones revealed the requirement of neuropeptide F, a homolog of the mammalian neuropeptide Y, for the action of SBT2227. These biogenic effects of SBT2227 on the host sleep provide new insights into the interaction between the brain and gut bacteria.

Subject areas

Microbiology
Microbiome
Omics

Data and code availability

The original/source data are available from the lead contact on request. This paper does not report original code. Any additional information required to reanalyze the data reported in this paper is available from the lead contact upon request.

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3

These authors contributed equally

4

Lead contact