iScience
Volume 24, Issue 1, 22 January 2021, 101968
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Article
Dopamine induces functional extracellular traps in microglia

https://doi.org/10.1016/j.isci.2020.101968Get rights and content
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open access

Highlights

  • Dopamine induces ETs in BV2 microglia and primary adult human microglia

  • Induced traps are independent of ROS, cell death, and actin polymerization

  • Microglia ETs are functional and can trap E. coli

  • Microglia ETs are also present in Glioblastoma multiforme

Summary

Dopamine (DA) plays many roles in the brain, especially in movement, motivation, and reinforcement of behavior; however, its role in regulating innate immunity is not clear. Here, we show that DA can induce DNA-based extracellular traps in primary, adult, human microglia and BV2 microglia cell line. These DNA-based extracellular traps are formed independent of reactive oxygen species, actin polymerization, and cell death. These traps are functional and capture fluorescein (FITC)-tagged Escherichia coli even when reactive oxygen species production or actin polymerization is inhibited. We show that microglial extracellular traps are present in Glioblastoma multiforme. This is crucial because Glioblastoma multiforme cells are known to secrete DA. Our findings demonstrate that DA plays a significant role in sterile neuro-inflammation by inducing microglia extracellular traps.

Subject areas

Molecular Biology
Cellular Neuroscience
Immunology
Cell Biology

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