Antibacterial activity of a polysaccharide produced from Chaetomium globosum CGMCC 6882
Introduction
As effective means to prevent and control disease and improve the efficiency of breeding, antibiotics are widely used in people's daily life, but the long-term and extensive use of antibiotics have promoted the development of strong resistance of pathogenic bacteria [1,2]. The bacterial resistance and the adverse side effects induced by excessive use of traditional antibiotic drugs have urged the search for new antibacterial agents [3,4]. Therefore, natural compounds from medicinal plants or microorganisms with antibacterial capacities have a high potential for developing as antimicrobial drugs.
Endophytic fungus, as a new kind of microbial resources and separated from plants, has attracted more and more attention due to its natural active products [5,6]. For instance, the paclitaxel produced by Yew endophytic fungus is one of the best natural anticancer drugs in clinical application [7]. The 4-anilinoquinoline derivatives produced by Dendrobium officinale endophytic fungus have good antitumor and antifungal activities [8]. Dibenzo-α-pyrones from the Salvia miltiorrhiza endophytic fungus not only possessed antibacterial effect, but also displayed promising antioxidant activities [9]. Diapolic acid from Diaporthe terebinthifolii endophytic fungus depicted antiviral, anticancer and antifungal activities [10]. The flavones chrysin extracted from the fermentation broth of Passiflora incarnata endophytic fungus has the effect of antifatigue and antiaging effects [11].
In this research, the monosaccharide composition, molecular weight, functional groups and chemical bonds of a polysaccharide produced from an endophytic fungus Chaetomium globosum CGMCC 6882 were analyzed. Meanwhile, the antibacterial activities of GCP against E. coli and S. aureus were also characterized, including inhibition zones, minimum inhibitory concentration (MIC), cell wall and inner membrane permeability, electrical conductivity and changes of bacterial morphology.
Section snippets
GCP production
C. globosum CGMCC 6882 was isolated from G. pentaphyllum herb root and stored in China General Microbiological Culture Collection Center (China). The seed medium used for C. globosum CGMCC 6882 was consisted of 20 g/L glucose, 5 g/L peptone, 3 g/L beef extract and 1 g/L yeast extract, and pH of the medium was controlled between 7.0 and 7.2. The culture conditions are as follows: 50 mL liquid volume in 500 mL flask; 150 r/min shaking speed; 28 °C culture temperature.
Fermentation medium contains
Isolation and purification of GCP
After the fermentation liquid was collected, filtered, centrifuged at 12,000 ×g for 30 min to remove mycelium and cells, de-proteinized by adding three volumes Sevag solution, precipitated by adding three volumes of cold 95% alcohol and kept at 4 °C overnight, washed with 75% cold alcohol, de-pigmented with AB-8 macroporous resin, dialysed against distilled water and further purified by Sepharose CL-6B column (2.5 × 60 cm). One purified and homogeneous polysaccharide (GCP) was obtained with
Conclusions
A polysaccharide (GCP) with molecular weight and polydispersity of 8.093 × 104 Da and 1.014 was successfully produced by an endophytic fungus C. globosum CGMCC 6882. GCP was composed of arabinose, galactose, glucose, xylose, mannose and glucuronic acid in a molar ratio of 0.64: 2.58: 23.53: 0.90: 2.47: 0.27. Meanwhile, GCP exhibited excellent antibacterial activities against S. aureus than E. coli, and the minimum inhibitory concentrations of GCP against E. coli and S. aureus were 1.75 mg/mL
Abbreviation
- GCP
polysaccharide produced by an endophytic fungus C. globosum CGMCC 6882 which was isolated from G. pentaphyllum
Acknowledgements
This work is supported by the Natural Science Foundation of Youth Support Plan of Henan University of Technology (2017QNJH10) and High Level Research Fund for Qualified People of Henan University of Technology (2017BS011), the Program for Innovative Research Team (in Science and Technology) in University of Henan Province (19IRTSTHN008), the Natural Science Foundation of Henan Province Education Department (19A180015) and the national first-class discipline program of Light Industry Technology
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