Evaluation of Oxidative stress, antioxidant enzymes and genotoxic potential of bisphenol A in fresh water bighead carp (Aristichthys nobils) fish at low concentrations☆
Graphical abstract
Section snippets
Authorship contribution
Rabia Akram: conducted the experiment, prepared the first version of the manuscript. Rehana Iqbal: involved in experimentation plan and execution of trial, edited the final version of manuscript. Riaz Hussain: involved in experimentation plan and execution of trial, prepared the first version of the manuscript, edited the final version of manuscript. Farhat Jabeen: edited the final version of manuscript. Muhammad Ali: edited the final version of manuscript.
Fish management
The current experimental trial was executed at laboratories of departments of Zoology (Life sciences) and Pathology (Veterinary sciences) at Islamia University of Bahawalpur. A total of 80 bighead carp (Aristichthys nobilis) having similar body mass (150–175g), age and length were procured from local fish breeding center district Bahawalnagar. All the fish were shifted to the laboratories using plastic bags containing suitable oxygen. The test specimens were kept in glass aquaria (25” L × 45”
Physical observations
In the present investigation, no mortality of bighead carp was observed in treated groups (B, C, D) with variant concentrations of bisphenol A throughout the trial. Different behavioral and clinical signs such as increased swimming, loss of equilibrium, bulging eyes, lesions on the body surface (black spots on body surfaces), rapid operculum movement, and secretion of mucus from gills and mouth, erratic swimming, loss of coordination, swimming in isolation, air gulping and lying on one side
Discussion
Bisphenol A is extensively used in industries for manufacturing of different materials including thermal paper, water pipes, epoxy resins, kitchen utensils, electronic equipment, toys, dental sealants and polycarbonate plastics. Due to continuous discharge from industries bisphenol A has become the most important and deleterious health threat for both terrestrial and aquatic life (Kim et al., 2018; Andujar et al., 2019; Yang et al., 2019). BPA being the major part of aquatic bodies induces
Conclusions
From the findings of current trial our results indicated that bisphenol A induced deleterious effects on red blood cells and different vital organs of bighead carp. Exposure of bighead carp to bisphenol A at 1000 μg/L and 1500 μg/L causes DNA damage in isolated blood lymphocyte, brain, gills, liver and kidneys cells. Moreover, bisphenol A causes increased oxidative stress and reduces antioxidant enzymes in brain, livers, kidneys and gills of bighead carp in a concentration and time dependent
Declaration of competing interest
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Acknowledgment
We thank Dr. Abdul Ghaffar for providing us laboratory for conduction of this research. We thank Naseem Khan Director fisheries department for providing the test specimens. We also thank Dr. Corrie Brown, College of Veterinary Medicine, The University of Georgia Athens, USA, GA 30602–7388 for her critical discussion and revision of this manuscript. The current experimental research is part of PhD thesis and was not funded by any public and commercial sectors.
References (87)
- et al.
Permethrin drastically affects the developmental cycle of the non-target slime mould Dictyosteliumdiscoideum
Chemosphere
(2018) - et al.
Cypermethr intoxication leads to histopathological lesions and induces inflammation and apoptosis in common carp (Cyprinuscarpio L.)
Chemosphere
(2017) - et al.
Bisphenol A induces reactive oxygen species generation in the liver of male rats
Toxicology
(2003) - et al.
Roundup exposure promotes gills and liver impairments, DNA damage and inhibition of brain cholinergic activity in the Amazon teleost fish Colossoma macropomum
Chemosphere
(2015) - et al.
Assay of catalases and peroxidases
Methods Enzymol.
(1955) - et al.
Juvenile exposure to bisphenol A promotes ovarian differentiation but suppresses its growth Potential involvement of pituitary follicle stimulating hormone
Aquat. Toxicol.
(2017) - et al.
Oxidative stress in the hybrid fish jundiara (Leiarius marmoratus x Pseudoplatystoma reticulatum) exposed to Roundup Original
Chemosphere
(2017) - et al.
Degradation of endocrine disruptor bisphenol A by ultrasound-assisted electrochemical oxidation in water
Ultrason. Sonochem.
(2017) - et al.
Bisphenol A exposure, effects, and policy: a wildlife perspective
J. Environ. Manag.
(2012) - et al.
Vitamin C attenuates biochemical and genotoxic damage in common carp (Cyprinus carpio) upon joint exposure to combined toxic concentrations of fipronil and buprofezin insecticides
Aquat. Toxicol.
(2018)
Biochemical effects of fipronil and its metabolites on lipid peroxidation and enzymatic antioxidant defense in tadpoles (Eupemphixnattereri: leiuperidae)
Ecotoxicol. Environ. Saf.
High through put spectrophotometric assay of reactive oxygen species in serum
Mutat. Res.
New insights into the metabolism and toxicity of bisphenol A on marine fish under long-term exposure
Environ. Pollut.
Clinico-hematological and tissue changes induced by butachlor in male Japanese quail (Coturnix japonica)
Pestic. Biochem. Physiol.
JAK and STAT members in channel catfish: identification, phylogenetic analysis and expression profiling after Edwardsiella ictaluri infection
Dev. Comp. Immunol.
Bisphenol a disrupts steroidogenesis and induces a sex hormone imbalance through c-Jun phosphorylation in leydig cells
Chemosphere
Characteristics of nonylphenol and bisphenol A accumulation by fish and implications for ecological and human health
Sci. Total Environ.
Bisphenol A content in fish caught in two different sites of the Tyrrhenian Sea (Italy)
Chemosphere
Hypothalamicpituitary-ovarian axis perturbation in the basis of bisphenol A (BPA) reproductive toxicity in female zebrafish (Danio rerio)
Ecotoxicol. Environ. Saf.
A simple technique for quantitation of low levels of DNA damage in individual cells
Exp. Cell Res.
Concentration-dependent effect of bisphenol-a on insulin signaling molecules in cardiac muscle of adult male rat
Chem. Biol. Interact.
Distributions of concentrations of bisphenol A in North American and European surface waters and sediments determined from 19 years of monitoring data
Chemosphere
Adverse effects of bisphenol A on Sertoli cell blood-testis barrier in rare minnow Gobiocypris rarus
Ecotoxicol. Environ. Saf.
Clastogenic and mutagenic effects of bisphenol A: an endocrine disruptor
Mutat. Res.
Toxic effects of bisphenol A on goldfish gonad development and the possible pathway of BPA disturbance in female and male fish reproduction
Chemosphere
The measurement of bisphenol A and its analogues, perfluorinated compounds in twenty species of freshwater and marine fishes, a time-trend comparison and human health based assessment
Mar. Pollut. Bull.
Bisphenol A induces oxidative stress-associated DNA damage in INS-1 cells
Mutat. Res. Genet. Toxicol. Environ. Mutagen
Accelerated biodegradation of BPA in water-sediment microcosms with bacillus sp. GZB and the associated bacterial community structure
Chemosphere
Bisphenol analogues in surface water and sediment from the shallow Chinese freshwater lakes: occurrence, distribution, source apportionment, and ecological and human health risk
Chemosphere
Assessment of bisphenol A alternatives in paper products from the Chinese market and their dermal exposure in the general population
Environ. Pollut.
The effect of di-2-ethylhexyl phthalate on inflammation and lipid metabolic disorder in rats
Ecotoxicol. Environ. Saf.
The beneficial effects of ascorbic acid during chlorpyrifos-induced oxidative stress and histopathological changes in Oreochromis spilurus
Toxicol. Environ. Health Sci.
Assessment of DNA damage and oxidative stress in juvenile Channa punctatus (Bloch) after exposure to multi-walled carbon nanotubes
Environ. Toxicol.
Bisphenol A analogues in food and their hormonal and obesogenic effects: a review
Nutrients
Maternal urinary bisphenol a concentration during midterm pregnancy and children’s blood pressure at age 4
Hypertension
Bisphenol a interferes with swine vascular endothelial cell functions
Can. J. Physiol. Pharmacol.
Enhancing the wellbeing of Tamil fishing communities (and government bureaucrats too): the role of ur panchayats along the coromandel coast, India
2018. Estrogenic effects of several BPA analogs in the developing zebra fish brain
Front. Neurosci.
Effects of bisphenol A on Foxl2 gene expression and DNA damage in adult viviparous fish goodea atripinnis 2020
J. Toxicol. Environ. Health A.
Chronic exposure to low concentration of bisphenol A impacts on the first round of spermatogenesis via SIRT1 modulation
Sci. Rep.
Endocrine disrupting mechanisms of polychlorinated biphenyls
Curr. Opin. Toxicol.
Bisphenol-A (BPA) alters plasma thyroid hormones and sex steroids in female Pakistani major carp (catla catla; cyprinidae)
Pak. Vet. J.
Toxic effects of bisphenol A on sexual and asexual reproduction in Hydra oligactis
Arch. Environ. Contam. Toxicol.
Cited by (66)
Nonspecific immune, histology and accumulation of marine worm, Urechis unicinctus in response to bisphenol A (BPA)
2024, Ecotoxicology and Environmental SafetyBisphenol A contamination in Hilsa shad and assessment of potential health hazard: A pioneering investigation in the national river Ganga, India
2024, Journal of Hazardous MaterialsPhysiological impact of personal care product constituents on non-target aquatic organisms
2023, Science of the Total Environment
- ☆
This paper has been recommended for acceptance by Maria Cristina Fossi.