Culture, cryobanking and passaging of karyotypically validated native Australian amphibian cells
Section snippets
Brief communication
Amphibians are experiencing a critical existence juncture, with approximately 32% of the currently recognised species under immediate threat [1]. The main driver of this crisis is human activity and the spread of chydridiomycosis [1]. Conservation programs involving habitat conservation and assisted reproduction techniques (ART) have demonstrated success in safeguarding many threatened species from imminent extinction. A greater number of complementary techniques, including cryobanking
Funding
Richard Mollard provided all funds for the materials and the execution of experimental procedures described in this study.
Conflicts of interest
The author is the owner of Amphicell Pty Ltd, an Australian native frog conservation advocacy aiming to safeguard the future of amphibian biodiversity within Australia.
Acknowledgments
The author thanks Deborah Pergolotti from Frog Safe Inc. (funded through public donations) for provision of tissues from deceased animals. The author also thanks Associate Professor Jean-Paul Scheerlinck for access to tissue culture facilities, and Dr Charlie Pagel for the use of microscopic equipment for karyotyping; both at the University of Melbourne's Department of Veterinary and Agricultural Sciences.
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