Cell Reports
Volume 17, Issue 9, 22 November 2016, Pages 2286-2298
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Article
LYVE1 Marks the Divergence of Yolk Sac Definitive Hemogenic Endothelium from the Primitive Erythroid Lineage

https://doi.org/10.1016/j.celrep.2016.10.080Get rights and content
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Highlights

  • LYVE1 is expressed in yolk sac endothelium and hematopoietic stem and progenitor cells

  • Lyve1-Cre marks yolk sac definitive hematopoiesis but bypasses primitive erythropoiesis

  • LYVE1 lineage cells initiate fetal liver definitive erythropoiesis

  • A subset of fetal and adult hematopoietic stem cells are derived from the LYVE1 lineage

Summary

The contribution of the different waves and sites of developmental hematopoiesis to fetal and adult blood production remains unclear. Here, we identify lymphatic vessel endothelial hyaluronan receptor-1 (LYVE1) as a marker of yolk sac (YS) endothelium and definitive hematopoietic stem and progenitor cells (HSPCs). Endothelium in mid-gestation YS and vitelline vessels, but not the dorsal aorta and placenta, were labeled by Lyve1-Cre. Most YS HSPCs and erythro-myeloid progenitors were Lyve1-Cre lineage traced, but primitive erythroid cells were not, suggesting that they represent distinct lineages. Fetal liver (FL) and adult HSPCs showed 35%–40% Lyve1-Cre marking. Analysis of circulation-deficient Ncx1−/− concepti identified the YS as a major source of Lyve1-Cre labeled HSPCs. FL proerythroblast marking was extensive at embryonic day (E) 11.5–13.5, but decreased to hematopoietic stem cell (HSC) levels by E16.5, suggesting that HSCs from multiple sources became responsible for erythropoiesis. Lyve1-Cre thus marks the divergence between YS primitive and definitive hematopoiesis and provides a tool for targeting YS definitive hematopoiesis and FL colonization.

Keywords

LYVE1
lineage tracing
hemogenic endothelium
yolk sac
definitive hematopoiesis
primitive hematopoiesis
fetal liver

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