Communication
Revealing HOCl burst from endoplasmic reticulum in cisplatin-treated cells via a ratiometric fluorescent probe

https://doi.org/10.1016/j.cclet.2020.12.039Get rights and content

Abstract

The reactive oxygen species (ROS) are tightly associated with endoplasmic reticulum (ER) stress. Thus, the deep and visual insight of aberrant ROS fluctuations in the ER can help us better investigate the ER stress-associated pathology. In this work, a fluorescent probe ERC for HOCl detection in the ER based on phenothiazine-derived coumarin platform was developed. In the presence of HOCl, ERC exhibited an emission change from 609 nm to 503 nm within seconds. It also showed high sensitivity (0.44 μmol/L) and superb photostability. Significantly, ERC displayed low cytotoxicity, good cell membrane permeability, and appreciable ER-targetability. Ultimately, the probe was successfully utilized to image exogenous and endogenous HOCl in living cells and reveal the HOCl burst in cisplatin-treated cancer cells.

Graphical abstract

An ER-targeted fluorescent probe was presented for the HOCl detection in cisplatin-stimulated cancer cells.

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Declaration of competing interest

The authors report no declarations of interest.

Acknowledgment

This work was supported by the Hubei Provincial Department of Education Science and Technology Research Projects (No. Q20182704).

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    These authors contributed equally to this work.

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