NoteThe O-polysaccharide of Escherichia coli O112ac has the same structure as that of Shigella dysenteriae type 2 but is devoid of O-acetylation: a revision of the S. dysenteriae type 2 O-polysaccharide structure
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Bacterial strains and isolation of lipopolysaccharides
S. dysenteriae type 2 (G1252) was from the Institute of Medical and Veterinary Science, Adelaide, Australia, and E. coli O112ac:K66:H− (CCUG 11414) was from the Culture Collection, University of Gothenburg, Sweden. Bacteria were grown to late log phase using a fermentor under constant aeration at 37 °C and pH 7.0 as described for S. dysenteriae type 4;6 bacterial cells were washed and dried. Lipopolysaccharides were isolated from dried bacterial cells by the phenol–water method14 in a yield of
Acknowledgments
This work was supported by the Russian Foundation for Basic Research (Project 05-04-48992), the Russian Science Support Foundation, the Council on Grants at the President of the Russian Federation for Support of Young Russian Scientists (MK-157.2007.4), the Swedish Research Council, the Swedish Agency for Research Cooperation with Developing Countries, Tianjin Municipal Special Fund for Science and Technology Innovation Grant 05FZZDSH00800, the NSFC Key Programs Grants 30530010 and 20536040,
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