Engineering small molecule specificity in nearly identical cellular environments
Graphical abstract
Differential expression of FKBP12 in mouse embryonic fibroblasts demonstrates selective detoxification of MTXSLF, a small molecule that binds either FKBP12 or DHFR but not both enzymes simultaneously.
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Acknowledgments
We thank Hank Bayle, Joe Arron, and the Crabtree lab for advice and materials, and Laura Banaszynski for valuable discussions. This research was supported by the NIH (GM 068589), and M.A.S. was supported by the Stanford NIH Medical Scientist Training Program.
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