Research review paperThree decades of nucleic acid aptamer technologies: Lessons learned, progress and opportunities on aptamer development
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Keywords
SELEX
Aptamer
SELEX library
Synthetic genetic polymers
Single-strand DNA preparation
PCR bias
Abbreviations
SELEX
Systematic Evolution of Ligands by Exponential enrichment
AEGIS
artificially expanded genetic information system
AFM
atomic force microscope
AON
antisense oligonucleotide
CMACS
continuous-flow magnetic activated chips
Ds
7-(2-thienyl) imidazo [4, 5-b] pyridine
EMSA
electrophoretic mobility shift assay
ECEEM
equilibrium capillary electrophoresis of equilibrium mixtures
ePCR
Emulsion PCR
FACS
fluorescence-activated cell sorter
FITC
Fluorescein isothiocyanate
Flu-Mag
Fluorescence-Magnetic
LNA
locked nucleic acid
MARAS
Magnetic-Assisted Rapid Aptamer Selection
NA
Nucleic acid
NRR
non-homologous random recombination
NGS
next generation sequencing
P
2-amino-8-(10-β-D-2-deoxyribofuranosyl)-imidazo[1,2-a]-1,3,5-triazin-4(8H)-one
PEG
polyethylene glycol
Px
2-nitro-4- propynylpyrrole
RAPID
RNA aptamer Isolation via dual-cycles
SweepCE
Sweeping capillary electrophoresis
SOMAmer
Slow off-rate modified aptamer
ssDNA
single-stranded DNA
TECS
target expressed on the cell surface
Thioaptamers
Thiophosphate-modified aptamers
UV-LEDIF
ultraviolet light-emitting diode-induced native fluorescence
Z
6-amino-5-nitro-3-(1'- β-D-2'-deoxyribofur-anosyl)-2(1H)-pyridone
μFFE
Micro Free Flow Electrophoresis
2′-FANA
2'-fluoroarabino nucleic acid
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These authors contributed equally to this work.
© 2018 The Authors. Published by Elsevier Inc.