Bioprospecting of marine microalgae from Kaohsiung Seacoast for lutein and lipid production
Graphical abstract
Section snippets
1. Introduction
Microalgae have become a potential platform for biological carbon-capturing and reduction of greenhouse gas emissions (Choi et al., 2019, Patel et al., 2021e). Moreover, it offers an environmentally benign 3rd generation feedstock for biofuels such as bioethanol, biodiesel, biomethane, etc. (Choi et al., 2019, Patel et al., 2020a, Patel et al., 2021b) and high-value products carotenoids, proteins, and other bioactive compounds (Patel et al., 2021a, Patel et al., 2021c, Marker, 2022). In recent
Materials
All the chemicals used for production and analytics were obtained from Sigma Aldrich (USA). Reagents used for assays were of analytical grade and extraction solvents such as methanol, ethanol, acetone, acetonitrile, dichloromethane, and diethyl ether were obtained from J.T baker and Honeywell (New Jersey USA).
Seawater sample collection and enrichment
Seawater samples used for prominent microalgal growth have been collected from various locations of Kaohsiung Seacoast, Nanzih district, Taiwan and marked as C1, F1, KH1, and E1. These
Analytical methods
A spectrophotometer (Model UV-1800, Shimadzu, Japan) was used to measure the absorbance at 750 nm (OD 750) of the microalgae cells. An LI-COR photometer (Model LI-250 light meter, USA) was used to measure light intensity. A digital pH meter (Hanna Instrument, HI 8314 model) was used to measure the pH of the culture broth.
(a) Biomass estimation: Gravimetric filtration was used to determine the dry cell weight (DCW) of each microalgae biomass sample. To determine the biomass weight, the final dry
Isolation, selection, and identification of potential microalgae isolate
From morphological and growth characterization, results were encouraging from all studied isolates. These marine microalgae isolates were fast-growing strains and yielding minimum biomass > 2.5 g L−1 dry cell weight (DCW) which is a good range, however, average biomass yields were close to 3 g L−1 and some strains were exceptionally impressive with DCW yield close or > 4 g l−1. Most of the microalgal products are positively correlated with biomass yields (Patel et al., 2019), hence higher
Conclusions
Bioprospecting of the potential marine microalgae was aimed for high-value product synthesis. Selected isolate Kh12 exploited under biorefinery concept for lutein and fatty acids extraction. Isolate Chlorella sorokiniana Kh12 cultivated under mixotrophic modes, especially at 2X-HT-9 k produced the maximum biomass (3.46 g L−1) and lutein (13.69 mg g−1) yields. HT is composed of primary-metal- components of photosystem-II. Among various tested solvents, methanol was best for extraction. Bead
CRediT authorship contribution statement
Anil Kumar Patel: Conceptualization, Data curation, Writing – original draft, Writing – review & editing, Visualization, Supervision. Akash Pralhad Vadrale: Conceptualization, Writing – original draft. Yi-Sheng Tseng: Validation, Supervision. Chiu-Wen Chen: Validation, Supervision. Cheng-Di Dong: Validation, Data curation, Visualization, Supervision. Reeta Rani Singhania: Conceptualization, Data curation, Writing – original draft, Writing – review & editing.
Declaration of Competing Interest
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Acknowledgment
AKP, RRS, CWC, and CDD would like to acknowledge the Taiwan MOST for funding support (Ref. No. 109-2222-E-992-002).
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