Lipid class composition of membrane and raft fractions from brains of individuals with Alzheimer's disease

https://doi.org/10.1016/j.bbrep.2019.100704Get rights and content
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Highlights

  • No change was found in the composition of lipid classes of brains with Alzheimer's disease.

  • The APOE ε4 allele did not affect lipid class composition of the brain membrane or rafts.

  • The enzymatic measurement of phospholipids is applicable to brain tissues.

Abstract

Perturbation of the homeostasis of brain membrane lipids has been implicated in the pathomechanism of Alzheimer's disease (AD). The ε4 allele of the apolipoprotein E gene (APOE) confers an increased risk, in a dosage-dependent manner, for brain amyloid-β accumulation and the development of sporadic AD. An effect of the APOE genotype on brain lipid homeostasis may underlie the AD risk associated with the ε4 allele. In this research, we examined an effect of APOE ε4 on the lipid class composition of crude membranes and raft-enriched fractions of brains. We applied enzymatic reaction-based methods for the quantification of phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidic acid, and sphingomyelin. Our results indicate that brain lipid class composition was neither significantly altered in AD subjects nor affected by the presence of the APOE ε4 allele.

Keywords

Alzheimer's disease
Apolipoprotein E
Lipid composition
Brain
Lipid raft
Neurodegeneration

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