Human adiponutrin is present in plasma as disulfide bond-dependent multimers.
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Human plasma adiponutrin circulates in the low nM range, estimated to 1.25–4 nM.
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Adiponutrin is secreted by HepG2 cells in the presence of oleate.
Abstract
The interest in adiponutrin stems from adiponutrin variant I148M, which is strongly associated to non-alcoholic fatty liver disease. Adiponutrin has to date been considered to be solely an intracellular protein, with a role in lipid metabolism in liver and adipose tissue. However, a physiologically relevant role for adiponutrin has not been found. The aim of this study was to investigate the presence of adiponutrin in human plasma, a new facet of adiponutrin research. We demonstrate that adiponutrin is present in plasma as disulfide-bond dependent multimers, estimated to circulate at a concentration of 1.25–4 nM. Experiments reveal that adiponutrin is released from HepG2 cells in the presence of oleate. The presence of adiponutrin in plasma makes it accessible for clinical investigations and use as a potential biomarker for metabolic disease.
Abbreviations
PNPLA
patatin-like phospholipase domain-containing protein
