Development of an all-in-one technology for glycan profiling targeting formalin-embedded tissue sections
Section snippets
Materials and methods
Protein extraction from tissue sections. Formalin-fixed paraffine-embedded tissue microarrays (each section with 1.5 mm diameter and 5 μm thickness) were purchased from Cybrdi, Inc. (Frederick, MD). They consisted of brain glioblastoma (21 cases of individual patients, each in triplicate), colon adenocarcinoma (grade III; 23 cases of individual patients, each in triplicate), colon adenocarcinoma (grades I + II; 21 cases of individual patients, each in triplicate; for the term “grade”, see ref. [16]
Construction of a method for multiplex glycan analysis targeting one-dot tissue sections
Since alteration of glycans is often associated with tumor progression, searching disease-associated glyco-markers to visualize changes in glycan structure using tissue specimens should provide useful information. A multiplex glycan analysis, targeting a relatively homogeneous area of a tissue section comprising heterogeneous carcinoma cells by means of lectins and antibodies, is a methodology with the potential to allow discovery of useful glyco-biomarkers. Although a lectin microarray
Acknowledgments
We thank N. Uchiyama, Y. Kubo, J. Murakami, and S. Koizumi for technical assistance. We also thank Y. Ikehara, T. Sato, and Y. Itakura for helpful discussion. This study was supported by New Energy and Industrial Technology Development Organization (NEDO) in Japan.
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