Biochemical and Biophysical Research Communications
The FBXW7 β-form is suppressed in human glioma cells
Section snippets
Materials and methods
Cell lines and normal tissues. A total of 84 human cancer cell lines were analyzed in this study; 21 lung cancer cell lines (1–87, 11–18, 86–2, ABC-1, H1299, LCSC#1, LCSC#2, LK79, LK87, Lu65, Lu99A, Lu99B, RERF-LC-MS, RERF-LC-OK, Sato T, S-2, SBC3, Sq-1, Sq19, VMRC-LCD, VMRC-LCP), 28 pancreatic cancer cell lines (PK-1, PK-8, PK-9, PK-12, PK-14, PK-16, PK-36, PK-45 P, PK-45 H, PK-47, PK-59, PK-65, PK-66, PK-67, PAN03JCK, PAN07JCK, PAN08JCK, PAN09JCK, PCI-6, PCI-19, PCI-24, PCI-35, PCI-43, PCI-55,
Mutation analyses
In this study, we analyzed a total of 84 human tumor cell lines originating from 10 different organs. Mutations of the entire exonic regions (exons α, β, and γ, corresponding to the first exon for each of the α-, β-, and γ-forms of the transcript, and the common exons 2 through 11) of the FBXW7 gene were sought by sequencing the genomic DNAs from these cells. One colon cancer cell line, SCC, harbored a novel nonsense mutation (R393X) in exon 7 (Fig. 1A). No signal corresponding to the normal
Discussion
FBXW7, the gene that encodes the F-box protein responsible for targeting cell cycle accelerators such as CCNE1, AURKA, and MYC for ubiquitin-mediated proteolysis [1], has been found to be mutated in a number of primary cancers and cancer-derived cell lines [5], [12], [13], [14], [15], [16], [36]. In this study, we searched for FBXW7 mutations in 84 cell lines derived from 10 organs and found mutations in two of 10 (20%) colon cancer cell lines and one of seven (14%) ovarian cancer cell lines.
Acknowledgments
We thank Dr. Barbara Lee Smith Pierce (Adjunct Professor with the University of Maryland University College) for editorial work in the preparation of this manuscript. This work was supported in part by Grants-in-Aid and the 21st Century COE Program Special Research Grant from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and by a Grant-in-Aid for Cancer Research from the Ministry of Health, Labour and Welfare of Japan.
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