Suppression of CaCYP1, a novel cytochrome P450 gene, compromises the basal pathogen defense response of pepper plants
Section snippets
Materials and methods
Plant materials and pathogen inoculation. Chili pepper (Capsicum annuum L. cv. Bukang) and the bell pepper (C. annum L. cv. Early Calwonder 30R;bs1/bs1, bs2/bs2, Bs3/Bs3) were used in the current study. Peppers were grown in a plant growth room at 24 ± 1 °C with a photoperiod of 16 h light. Six weeks after seed germination, pepper plants were treated with bacterial pathogens or various chemicals. The bacterial pathogens used for inoculation were X. axonopodis pv. glycines 8ra (Xag 8ra), a soy bean
Isolation of pepper CaCYP1 using cDNA microarray analysis following non-host pathogen infection
To isolate pepper genes induced during the non-host bacterial pathogen HR, a pepper cDNA microarray was probed with RNA extracted from Xag8ra-infected hot pepper leaves. Xag8ra is not a pathogen of pepper, but does elicit an HR in pepper leaves, as well as induce the expression of a number of PR genes [30]. Microarray analysis indicated that 40 (out of 350 total ESTs) gene sequences were up-regulated more than 2-fold following Xag infiltration. This result was subsequently confirmed by reverse
Acknowledgments
This work was supported by the 21st frontier research programs of MOST, Crop Functional Genomics Center, Plant Diversity Research Center and in part by PMGBRC, one of the KOSEF science research Center.
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