Selective inhibition of bleomycin-induced G2 cell cycle checkpoint by simaomicin α
Section snippets
Materials and methods
Materials. Simaomicin α was isolated from the culture broth of Actinomadura sp. KA-6744. Xanthoquinodins and cervinomycins (Fig. 1) were isolated from culture broths of Humicola sp. FO-888 [24] and Streptomyces cervinus AM-5344 [25], respectively. Jurkat cells were kindly provided by the Cell Resource Center for Biomedical Research, Tohoku University, Japan. Human umbilical vein endothelial cells (HUVEC) were purchased from Dainippon Pharmaceutical (Osaka, Japan). Fetal calf serum (FCS) was
Inhibition of bleomycin-induced G2 arrest in Jurkat cells by simaomicin α
Jurkat cells were cultured for 20 h and the cell cycle status was analyzed. The distribution ratios in subG1, G1, S, and G2/M phases were 4.5%, 51.9%, 21.9%, and 21.2%, respectively (Fig. 2C). When Jurkat cells were incubated in the presence of simaomicin α (0.6–6.0 nM), the distribution ratios in subG1, G1, S, and G2/M phases were almost fundamentally unchanged; 4.7–10.1%, 47.2–49.7%, 21.5–23.2%, and 15.2–20.2% (Fig. 2C), respectively. These data indicated that simaomicin α alone shows no effect
Acknowledgements
This study was supported in part by a grant from the 21st Century COE Program, Ministry of Education, Culture, Sports, Science and Technology, Japan.
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