Biochimica et Biophysica Acta (BBA) - Biomembranes
Volume 1828, Issue 2, February 2013, Pages 357-364
Effect of oxidative stress on plasma membrane fluidity of THP-1 induced macrophages
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Graphical abstract
Highlights
► Oxidative stress causes an increase in macrophage plasma membrane rigidity. ► H2O2 causes an increase in the number, but not the size, of raft domains. ► Macrophage is highly resistant to H2O2 as viability, in our conditions, is above 80%. ► New raft domains in macrophages might be involved in cell survival pathways.
Abbreviations
AAPH
2,2′‐azobis(2‐amidinopropane) dihydrochloride
DMSO
dimethylsulphoxide
DPPC
dipalmitoylphosphatidylcholine
FBS
fetal bovine serum
GP
generalized polarization
GUVs
giant unilamellar vesicles
ld
liquid disordered
lo
liquid ordered
MTT
3‐[4,5‐dimethylthiazol‐2‐yl]‐2,5‐diphenyltetrazolium bromide
PMA
phorbol‐12‐myristate‐13‐acetate
ROS
reactive oxygen species
THP‐1
human acute monocytic leukemia cell line
Keywords
Oxidative stress
Macrophage
Membrane fluidity
Laurdan
Two-photon microscopy
Lipid raft
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