ORIGINAL ARTICLEThe effect of drug loading and multiple administration on the protein corona formation and brain delivery property of PEG-PLA nanoparticles
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Graphical abstract
Drug loading and multiple dosing affect the composition of protein corona. Complement proteins, immunoglobulins, RAB5A and CD36 in protein corona influence nanoparticles uptake in brain endothelial cells and microglia.
KEY WORDS
Protein corona
Nanoparticles
Brain delivery
Microglia
PEG-PLA
Abbreviations
αM
α-mangostin
Aβ
amyloid beta
BBB
blood‒brain barrier
CNS
central nervous system
cou6
coumarin 6
cou6-NPs
cou6-labeled nanoparticles
cou7
coumarin 7
DLS
dynamic light scattering
HCS
high content screening
LDLR
low density lipoprotein receptor
MCI
mild cognitive impairment
NP
blank PEG-PLA nanoparticles
NP-αM
αM-loaded PEG-PLA nanoparticles
NP-corona complexes
nanoparticle-corona complexes
PEG-PLA
poly(ethylene glycol)–poly(l-lactide)
TEER
trans-epithelial electrical resistance
TEM
transmission electronic microscope
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Peer review under responsibility of Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences.
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These authors made equal contributions to this work.
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