Elsevier

Algal Research

Volume 35, November 2018, Pages 449-461
Algal Research

Genomic characterization reveals significant divergence within Chlorella sorokiniana (Chlorellales, Trebouxiophyceae)

https://doi.org/10.1016/j.algal.2018.09.012Get rights and content
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open access

Highlights

  • High quality genomes of three Chlorella sorokiniana strains were sequenced.

  • Gene content and nucleotide identity are highly variable between the three characterized strains

  • A large complement of sex/flagellar related genes suggests the capacity for sexual reproduction.

  • Distinctive epigenetic machinery suggests potential variability in transcriptional regulation.

Abstract

Selection of highly productive algal strains is crucial for establishing economically viable biomass and bioproduct cultivation systems. Characterization of algal genomes, including understanding strain-specific differences in genome content and architecture is a critical step in this process. Using genomic analyses, we demonstrate significant differences between three strains of Chlorella sorokiniana (strain 1228, UTEX 1230, and DOE1412). We found that unique, strain-specific genes comprise a substantial proportion of each genome, and genomic regions with >80% local nucleotide identity constitute <15% of each genome among the strains, indicating substantial strain specific evolution. Furthermore, cataloging of meiosis and other sex-related genes in C. sorokiniana strains suggests strategic breeding could be utilized to improve biomass and bioproduct yields if a sexual cycle can be characterized. Finally, preliminary investigation of epigenetic machinery suggests the presence of potentially unique transcriptional regulation in each strain. Our data demonstrate that these three C. sorokiniana strains represent significantly different genomic content. Based on these findings, we propose individualized assessment of each strain for potential performance in cultivation systems.

Keywords

Chlorella sorokiniana
Genome analysis
Meiosis
DNA methylation
Polyketide synthetase (PKS)

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