Original investigationUltrasmall Superparamagnetic Iron-Oxide–enhanced MR Imaging of Normal Bone Marrow in Rodents: Original Research: Original Research1
Section snippets
Animals and Animal Procedures
The study was approved by the committee on animal research of our institution. Twenty 4-week-old female Sprague-Dawley rats (Charles River Laboratories Inc. Wilmington, MA) with a body weight of 150 g initially were included in the study. Of these, two rats were excluded from evaluation because they died during the MR imaging examination. The remaining 18 rats were randomly assigned to one of three groups: six rats each were administered an intravenous injection of either ferumoxtran-10,
Qualitative Results
On early T1-weighted SPGR images, up to 2 hours PI, all three contrast agents showed intense signal enhancement (+2) of the femoral bone marrow (Figure 1). At 24 hours PI, no remaining T1 signal enhancement (0) was observed for all rats after SHU555C administration and all rats after ferumoxytol administration, as well as 50% (3/3) of evaluated rats after ferumoxtran-10 administration. Bone marrow in three other rats showed slight persistent ferumoxtran-10 T1 enhancement (+1).
On early
Discussion
Data from this study show that the three USPIO contrast agents, ferumoxtran-10, ferumoxytol, and SHU555C, can be applied equally for T1- and T2-weighted MR imaging of bone marrow. USPIO-enhanced early postcontrast T1-weighted MR images provide marked positive bone marrow enhancement because of a perfusion effect, whereas delayed postcontrast T2-weighted images provide marked negative bone marrow enhancement because of iron oxide uptake in bone marrow macrophages. In rodents, this negative bone
References (31)
- et al.
Physical and chemical properties of superparamagnetic iron oxide MR contrast agentsferumoxides, ferumoxtran, ferumoxsil
Magn Reson Imaging
(1995) - et al.
First-pass and equilibrium phase MRA following intravenous bolus injection of SHU555Cphase I clinical trial in elderly volunteers with risk factors for arterial vascular disease
Acad Radiol
(2002) - et al.
Superparamagnetic iron oxides as positive MR contrast agentsin vitro and in vivo evidence
Magn Reson Imaging
(1993) - et al.
Clinical and prognostic significance of femoral marrow magnetic resonance imaging in patients with malignant lymphoma
Blood
(1997) - et al.
[The diagnostic problems in magnetic resonance tomography of the bone marrow in patients with malignomas under G-CSF therapy]
Radiologe
(2000) - et al.
Red marrow recolonization induced by growth factors mimicking an increase in tumor volume during preoperative chemotherapyMR study
J Comput Assist Tomogr
(1997) - et al.
Relationship between bone marrow cellularity and apparent diffusion coefficient
J Magn Reson Imaging
(2001) - et al.
Initial experience with dynamic MR imaging in evaluation of normal bone marrow versus malignant bone marrow infiltrations in humans
J Magn Reson Imaging
(1997) - et al.
MRI gadolinium enhancement of bone marrowage-related changes in normals and in diffuse neoplastic infiltration
Skeletal Radiol
(1997) - et al.
Bone marrowultrasmall superparamagnetic iron oxide for MR imaging
Radiology
(1991)