Fluorescence detection of adenosine triphosphate using smart probe

doi:10.1016/j.ab.2012.06.022

Analytical Biochemistry

Volume 429, Issue 1, 1 October 2012, Pages 8-10

https://doi.org/10.1016/j.ab.2012.06.022 Get rights and content

Abstract

A novel fluorescent probe for adenosine triphosphate (ATP) assay based on DNA ligation is proposed in this article. This approach uses a novel smart probe, T4 DNA ligase, and two short oligonucleotides. In the presence of ATP, the T4 DNA ligase catalyzes the ligation reaction and the ligation product restores the fluorescence of the smart probe. This method is very sensitive with a 0.5-nM limit of detection. Compared with current assay methods, the strategy is simpler, cheaper, and 40 times more sensitive.

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Acknowledgments

This work was supported by the Fundamental Research Funds for the Central Universities (2012QNZT035). C. Ma thanks the Ames Laboratory for partial support of this work. The authors thank Edward S. Yeung for valuable discussions.

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Notes & TipsFluorescence detection of adenosine triphosphate using smart probe

Abstract

Section snippets

Acknowledgments

Biochim. Biophys. Acta

J. Neurosci. Methods

Food Prot.

Biosens. Bioelectron.

Curr. Opin. Chem. Biol.

Anal. Biochem.

Anal. Biochem.

Anal. Biochem.

Cellular Energy Metabolism and Its Regulations

Cystic fibrosis transmembrane conductance regulator and adenosine triphosphate

Science

Intracellular ATP levels determine cell death fate by apoptosis or necrosis

Cancer Res.

Genesis and reversal of the ischemic phenotype in epithelial cells

J. Clin. Invest.

Direct observation of calcium-independent intercellular ATP signaling in astrocytes

Anal. Chem.

Firefly bioluminescent assay of ATP in the presence of ATP extractant by using liposomes

Anal. Chem.

Immobilization of luciferase from a firefly lantern extract on glass strips as an alternative strategy for luminescent detection of ATP

J. Biolumin. Chemilumin.

Determination of adenosine triphosphoric acid by its effect on the quenching of the fluorescence of europium diketonate in micelles of Brij-35

J. Anal. Chem.

Aptamer-based optical probes with separated molecular recognition and signal transduction modules

J. Am. Chem. Soc.

Notes & Tips
Fluorescence detection of adenosine triphosphate using smart probe