Chapter Three - Membrane-Mimetic Inverse Bicontinuous Cubic Phase Systems for Encapsulation of Peptides and Proteins
Section snippets
Inverse Bicontinuous Cubic Phases
The inverse bicontinuous cubic phases (QII phases) of amphiphile-aqueous solution systems, have found application in the fields of soft materials, biochemistry, physical chemistry, and biotechnology due to their unique properties and are increasingly used for encapsulation of proteins and peptides [1]. In particular these phases are being used for in meso crystallization and drug delivery [2], [3], [4], both of which will be discussed later in the chapter. The inverse bicontinuous cubic phases
Peptide and Protein Encapsulation: Understanding the Structural Relationship Between Guest Molecules and the Cubic Phase
End-use applications of the lipidic cubic phase, whether in biochemistry, biotechnology, or drug delivery, often depend upon the successful encapsulation of proteins or peptides. However, the complex structural relationship between the individual components of the system must be well understood if we are to make full use of these materials (Fig. 2). First, the mesostructure of the cubic phase is heavily dependent upon the chemical structure of individual lipid molecules, reflected in factors
Lipid Packing, Interfacial Curvature, and Lateral Pressure
Physicochemical parameters of the lipid mesophase, including lipid packing, interfacial curvature, and lateral pressure, significantly affect its ability to encapsulate proteins and peptides [34], [35]. The structure and geometry of self-assembled lipid phases, in particular the intrinsic monolayer curvature, are determined predominately by the three-dimensional structure and shape of the lipid itself, which can be expressed in terms of the CPP from Israelachvili and Mitchell [36] and
Applications of Bicontinuous Cubic Phases for Protein or Peptide Encapsulation
The encapsulation of proteins and peptides, and the structural effect they exert on the bicontinuous cubic phase is of particular importance when considering their end-use applications, which typically rely on retention of specific cubic phase symmetries, as well as reliable encapsulation of peptides and proteins, often with retention of specific conformations and preservation of activity. Therefore, we briefly describe the two main applications of such materials, in meso crystallization and
Cubic Phase Nanoparticles (Cubosomes)
The coexistence of the inverse bicontinuous cubic phase in excess water allows for the production of cubic phase nanoparticles. First reported in 1989, these nanoparticles, termed cubosomes, consist of small lipid-based nanoparticles typically ~ 100–400 nm, which retain both the triply periodic minimal surface geometry of the bulk cubic phase as well as the ability to encapsulate peptides and proteins [25]. As such, they broaden the range of applications for the protein-encapsulated lipidic cubic
Characterization of Bicontinuous Cubic Phase-Peptide/Protein Systems
Most studies characterizing protein encapsulation within the cubic phase have relied heavily on the technique of small angle X-ray scattering (SAXS). Its great utility stems from the fact that in aqueous lipid samples both the phase and the lattice parameter, a measure of unit cell size, can be determined with little effort [81]. Resolution and analysis time can be improved through the use of high flux synchrotron radiation, as well as high-throughput methodologies [74], [82], [83], [84], [85].
Conclusion
The encapsulation of peptides and proteins in the lipidic bicontinuous cubic phase has been demonstrated to induce a number of structural changes, which affect the material properties for end-use applications, while also providing broader information regarding the interactions between biomolecules and the lipid bilayer. The nature of these structural changes is highly dependent on lipid and bilayer properties, including the hydrophobic lipid chain size, resulting bilayer thickness and, by
Acknowledgments
T.G.M. was the recipient of a CSIRO-Melbourne Research Scholarship. Figures were produced with the assistance of MarvinSketch 16.8.8, 2016, ChemAxon (http://www.chemaxon.com); Qutemol 0.4.1, 2007 [129]; K3DSurf 0.6.2, 2007; and Blender 2.77, 2016.
References (129)
- et al.
Surfactant self-assembly objects as novel drug delivery vehicles
Curr. Opin. Colloid Interface Sci.
(1999) Protein interactions and membrane geometry
Biophys. J.
(2003)Phase behavior of a monoacylglycerol: (myverol 18–99K)/water system
Chem. Phys. Lipids
(2000)- et al.
The phase diagram of the monoolein/water system: metastability and equilibrium aspects
Biomaterials
(2000) Monoacylglycerols: the workhorse lipids for crystallizing membrane proteins in mesophases
Mem. Protein Crystal.
(2009)Progress in liquid crystalline dispersions: cubosomes
Curr. Opin. Colloid Interface Sci.
(2005)Incorporation of antimicrobial peptides in nanostructured lipid membrane mimetic bilayer cubosomes
Colloids Surf B Biointerfaces
(2017)- et al.
A model for the packing of lipids in bilayer membranes
Biochim. Biophys. Acta
(1975) Turning protein crystallisation from an art into a science
Curr. Opin. Struct. Biol.
(2004)Overcoming the challenges of membrane protein crystallography
Curr. Opin. Struct. Biol.
(2008)
Membrane protein crystallization
J. Struct. Biol.
A lipid-based liquid crystalline matrix that provides sustained release and enhanced oral bioavailability for a model poorly water soluble drug in rats
Int. J. Pharm.
Lyotropic liquid crystalline phases formed from glycerate surfactants as sustained release drug delivery systems
Int. J. Pharm.
Cubic phase gels as drug delivery systems
Adv. Drug Deliv. Rev.
Liquid crystalline phases of monoolein and water for topical delivery of cyclosporin A: characterization and study of in vitro and in vivo delivery
Eur. J. Pharm. Biopharm.
Drug delivery from a liquid crystalline base across visking and human stratum corneum
Int. J. Pharm.
Buccal permeation of [D-Ala 2, D-Leu 5] enkephalin from liquid crystalline phases of glyceryl monooleate
Int. J. Pharm.
Controlling release from the lipidic cubic phase. Amino acids, peptides, proteins and nucleic acids
J. Control. Release
Embedding DNA in surfactant mesophases: the phase diagram of the ternary system dodecyltrimethylammonium–DNA/monoolein/water in comparison to the DNA-free analogue
J. Colloid Interface Sci.
Cationic lipid nanosystems as carriers for nucleic acids
New Biotechnol.
Preparation of phytantriol cubosomes by solvent precursor dilution for the delivery of protein vaccines
Eur. J. Pharm. Biopharm.
Steric stabilizers for cubic phase lyotropic liquid crystal nanodispersions (cubosomes)
Adv. Planar Lipid Bilayers Liposomes
Protein entrapment in PEGylated lipid nanoparticles
Int. J. Pharm.
Disposition and association of the steric stabilizer Pluronic® F127 in lyotropic liquid crystalline nanostructured particle dispersions
J. Colloid Interface Sci.
Characterization and potential applications of nanostructured aqueous dispersions
Adv. Colloid Interf. Sci.
Self-assembled nano-architecture liquid crystalline particles as a promising carrier for progesterone transdermal delivery
Int. J. Pharm.
Amphipathic polyethyleneglycols effectively prolong the circulation time of liposomes
FEBS Lett.
Characterization of lipid matrices for membrane protein crystallization by high-throughput small angle X-ray scattering
Methods
High resolution 1H NMR of a lipid cubic phase using a solution NMR probe
J. Magn. Reson.
Lipid and water diffusion in bicontinuous cubic phases measured by NMR
Biophys. J.
Stabilization of insulin against agitation-induced aggregation by the GMO cubic phase gel
Int. J. Pharm.
Gramicidin structure and disposition in highly curved membranes
J. Struct. Biol.
Cryogenic transmission electron microscopy (cryo-TEM) for studying the morphology of colloidal drug delivery systems
Int. J. Pharm.
Effect of sucrose on the structure of a cubic phase formed from a monoolein/water mixture
J. Colloid Interface Sci.
Nanostructured bicontinuous cubic lipid self-assembly materials as matrices for protein encapsulation
Soft Matter
Advances in drug delivery and medical imaging using colloidal lyotropic liquid crystalline dispersions
J. Colloid Interface Sci.
Crystallizing membrane proteins for structure determination: use of lipidic mesophases
Annu. Rev. Biophys.
Greasing membrane fusion and fission machineries
Traffic
Watching fat digestion
Science
The Language of Shape
Ordered 2-D and 3-D nanostructured amphiphile self-assembly materials stable in excess solvent
Phys. Chem. Chem. Phys.
Lyotropic liquid crystal engineering-ordered nanostructured small molecule amphiphile self-assembly materials by design
Chem. Soc. Rev.
Monoolein: a magic lipid?
Phys. Chem. Chem. Phys.
The temperature-composition phase diagram and mesophase structure characterization of the monoolein/water system
J. Phys. II
Phase behavior of the phytantriol/water system
Langmuir
Soft ordered mesoporous materials from nonionic isoprenoid-type monoethanolamide amphiphiles self-assembled in water
Soft Matter
Monodisperse nonionic phytanyl ethylene oxide surfactants: high throughput lyotropic liquid crystalline phase determination and the formation of liposomes, hexosomes and cubosomes
Soft Matter
Monoolein: a review of the pharmaceutical applications
Drug Dev. Ind. Pharm.
Cancer-cell-targeted theranostic cubosomes
Langmuir
Cubosomes as targeted drug delivery systems—a biopharmaceutical approach
Curr. Drug Discov. Technol.
Cited by (6)
Impact of pasteurization on the self-assembly of human milk lipids during digestion
2022, Journal of Lipid ResearchExploration of the cardinal formulation parameters influencing the encapsulation and physicochemical properties of co-loaded anticancer dual drug nanoliposomes
2022, Journal of Drug Delivery Science and TechnologyNanoscale structural and mechanical characterization of thin bicontinuous cubic phase lipid films
2022, Colloids and Surfaces B: BiointerfacesInteraction of Metallic Nanoparticles With Biomimetic Lipid Liquid Crystalline Cubic Interfaces
2022, Frontiers in Bioengineering and BiotechnologyLiquid Organic Frameworks: The Single-Network "plumber's Nightmare" Bicontinuous Cubic Liquid Crystal
2020, Journal of the American Chemical Society