Document heading
In vitro callus induction and plantlet regeneration of Achyranthes aspera L., a high value medicinal plant

https://doi.org/10.1016/S2221-1691(14)60206-9Get rights and content

Abstract

Objective

To study callus induction from different explants (internode, leaf, root) and in vitro plantlets propagation from medicinally important plant Achyranthes aspera L.

Methods

Sterilized explants were prepared by using 0.1% HgCl2 and 0.5% Bavistin and callus was obtained when cultured onto Murashige Skoog's (MS) medium by using different concentrations and combination of 2,4-D, NAA, BAP, IAA, IBA with 3% sucrose and 0.8% agar. Induced callus was immediately transferred to MS medium containing at different concentrations of phytohormones for shootlets and rootlets induction respectively.

Results

Sterilization treatment of 0.1% HgCl2 for 2–3 min and Bavistin 0.5% for 10–12 min showed the highest percentage of asepsis and survival rate. Maximum induction of callus was obtained from a combination of 2.0 mg/L 2,4-D and 0.5 mg/L NAA from leaf. Highest shootlets number (4.83±0.17) and length (3.8±0.16) cm were observed on full strength MS medium when fortified with BAP 4.0 mg/L and KIN 0.5 mg/L. Concerted efforts of BAP 2.0 mg/L and NAA 0.5 mg/L on full strength MS medium showed highest leaf number (6.77±0.94). In vitro raised shoots were allowed to root on different strengths of MS medium fortified with IAA and IBA at different concentrations. Experimentally, 3.0 mg/L IBA was enabled to induce maximum rootlets number (10.0±9.82) on full strength MS medium. Afterwards, regenerated shoots with well developed roots were successfully subjected to hardening process and were acclimatized. The survived plantlets showed 66.67% survival frequency without any morphological abnormality.

Conclusions

The results demonstrated that different explants were good source of callus induction, morphology analysis as well as indirect plantlets regeneration.

References (23)

  • M Johnson et al.

    Antibacterial activity of leaves and inter-nodal callus extracts of Mentha arvensis L

    Asian Pac J Trop Med

    (2011)
  • KV Saritha et al.

    In vitro flowering of Withania somnifera Dunal.-An important antitumor medicinal plant

    Plant Sci

    (2007)
  • S Srivastav et al.

    Achyranthes aspera: An important medicinal plant: A review

    J Nat Prod Plant Resour

    (2011)
  • D Sumeet et al.

    Achyranthes aspera Linn.(Chirchira): A magic herb in folk medicine

    Ethnobotanical Leaflets

    (2008)
  • R Londonkar et al.

    Potential antibacterial and antifungal activity of Achyranthes aspera L

    Recent Res Sci Technol

    (2011)
  • MK Sen et al.

    Phytochemistry and antimicrobial potential of apang (Achyranthes aspera L.): An update review

    Res Rev J Pharm Sci

    (2012)
  • AJ Khandagle et al.

    Bioactivity of essential oils of Zingiber officinalis and Achyranthes aspera against mosquitoes

    Parasitol Res

    (2011)
  • R Kartik et al.

    Amelioration effects against N-nitrosodiethylamine and CCl4-induced hepatocarcinogenesis in Swiss albino rats by whole plant extract of Achyranthes aspera

    Indian J Pharmacol

    (2010)
  • T Malarvili et al.

    Effect of Achyranthes aspera (Linn) seeds on redox and oxidative status in plasma and selected tissues of rats fed with high doses of fructose

    Biosci Biotechnol Res Asia

    (2009)
  • A Gomes et al.

    Herbs and herbal constituents active against snake bite

    Indian J Exp Biol

    (2010)
  • S Alkari et al.

    Effect of Achyranthes aspera on Helicoverpa armigera

    Ann Plant Protect Sci

    (2009)

    • Cited by (52)

    • Development of an efficient in vitro regeneration system in Costus speciosus - an important herbal insulin plant

      2022, South African Journal of Botany
      Citation Excerpt :

      Further, results described by Kou et al. (2013) were also in assent with the current findings of using these synthetic PGRs in culture medium, and also the low concentration of NAA in integration with a high concentration cytokinins resulted in induction of callus in Curcuma attenuate. Some other studies showed the successive callus induction on important medicinal plants such as Solanum nigrum (Sridhar and Naidu, 2011), Achyranthes aspera (Sen et al., 2014), Satureja khuzestanica (Ghorbanpour and Hadian, 2015), Thymus persicus (Bakhtiar et al., 2016), Lantana camara (Veraplakorn, 2016), and Stevia rebaudiana (Keshvari et al., 2018). After 5 - 6 weeks of callus induction, well-developed greenish proliferated calli were grown in the shooting medium reinforced with several combinations and doses of cytokinins and auxins.

    • Role of reduced nitrogen for induction of embryogenic callus induction and regeneration of plantlets in Abelmoschus esculentus L.

      2020, South African Journal of Botany
      Citation Excerpt :

      Cotyledon-derived calli showed only 49.50% responses on 1.5 mgL−1 BAP + 0.5 mgL−1 NAA. Similarly, the highest shoot regeneration response of BAP and NAA in Achyranthes aspera were reported (Sen et al., 2014). Additionally, it was demonstrated that variation in shoot number may be due to the nitrogen supply or the meristem induced (Ramage and Williams, 2002).

    • Conservation and sustainable use of medicinal plants

      2020, Preparation of Phytopharmaceuticals for the Management of Disorders: The Development of Nutraceuticals and Traditional Medicine

    • An Efficient Agrobacterium-Mediated Genetic Transformation System for Gene Editing in Strawberry (Fragaria × ananassa)

      2024, Plants

    • An Optimized Protocol for Seed Sterilization and Shoot Regeneration from Mature Embryo in Wheat (Triticum Aestivum L.) var. HD2967

      2023, Letters in Applied NanoBioScience

    • Picloram enhanced the callus induction, growth kinetics, antioxidant potentials, and secondary metabolites production of Zingiber officinale var. rubrum callus cultures

      2023, Plant Cell, Tissue and Organ Culture
    View all citing articles on Scopus

    Foundation Project: Supported by Islamic University, Kushtia−7003, Bangladesh (Grant No. IUBT-1108).

    Available online 28 Jan 2014

    Peer reviewer Dr. Rezuanul Islam, Associate Professor, Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia−7003, Bangladesh. E-mail: [email protected]

    Comments In this study, an effort has been made for in vitro callus induction and micropropagation for medicinally important plant A. aspera. The data presented in results and discussion of this work is interesting.

    Details on Page 45

    View full text
    Copyright © 2014 Asian Pacific Tropical Biomedical Magazine. Published by Elsevier (Singapore) Pte Ltd. All rights reserved.