The polysaccharide isolated from Pleurotus nebrodensis (PN-S) shows immune-stimulating activity in RAW264.7 macrophages

https://doi.org/10.1016/S1875-5364(15)30026-1Get rights and content

Abstract

A novel Pleurotus nebrodensis polysaccharide (PN-S) was purified andcharacterized, and its immune-stimulating activity was evaluated in RAW264.7 macrophages. PN-S induced the proliferation of RAW264.7 cells in a dose-dependent manner, as determined by the MTT assay. After exposure to PN-S, the phagocytosis of the macrophages was significantly improved, with remarkable changes in morphology being observed. Flow cytometric analysis demonstrated that PN-S promoted RAW264.7 cells to progress through S and G2/M phases. PN-S treatment enhanced the productions of interleukin-6 (IL-6), nitric oxide (NO), interferon gamma (INF-γ), and tumor necrosis factor-α (TNF-α) in the macrophages, with up-regulation of mRNA expressions of interleukin-6 (IL-6), inducible nitric oxide synthase (iNOS), interferon gamma(INF-γ) and tumor necrosis factor-α (TNF-α) being observed in a dose-dependent manner, as measured by qRT-PCR. In conclusion, these results suggest that the purified PN-S can improve immunity by activating macrophages.

Pleurotus nebrodensis polysaccharide (PN-S) can coordinate innate immunity with activating RAW264.7 macrophages.

  1. Download : Download full-size image

Cited by (29)

  • Understanding immune-modulatory efficacy in vitro

    2022, Chemico-Biological Interactions
    Citation Excerpt :

    They are then fixed in 70% glacial ethanol, washed in PBS, resuspended in 1 ml of PBS containing 50 U/ml RNase and 50 μg/ml propidium iodide (PI) and then incubated in dark for 40 min at 4 °C. Cell cycle analysis is performed by flow cytometry and the population of cells in various phases are calculated [51]. After encountering stimuli, macrophages are induced to undergo morphodynamics such as increase in cell size, production of thin sheets of cell edges called filopodia (contains actin filaments) or lamellopodia (bears actin spikes) [21].

  • Classification, structure and mechanism of antiviral polysaccharides derived from edible and medicinal fungus

    2021, International Journal of Biological Macromolecules
    Citation Excerpt :

    Most recently, Cui and colleagues demonstrated that the phagocytosis of macrophages was markedly improved after exposure to PN-S, with observed remarkable changes in morphology. PN-S was reported to improve RAW264.7 cells to progress via S and G2/M phases [196,197]. Additionally, PN-S could enhance the production of IL-6, NO, INF-γ, and TNF-α in the macrophages, with up-regulating mRNA expressions of IL-6, iNOS, INF-γ, and TNF-α being monitored in a dose-dependent manner.

View all citing articles on Scopus

Available online 20 May 2015

Research funding This work was supported by the National Natural Science Foundation of China (Nos. 31101357 and 31271915).

These authors have no conflict of interest to declare.

View full text