Soil enzymatic activities and microbial community structure with different application rates of Cd and Pb

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Abstract

This study focused on the changes of soil microbial diversity and potential inhibitory effects of heavy metals on soil enzymatic activities at different application rates of Cd and/or Pb. The soil used for experiments was collected from Beijing and classified as endoaquepts. Pots containing 500 g of the soil with different Cd and/or Pb application rates were incubated for a period of 0, 2, 9, 12 weeks in a glasshouse and the soil samples were analyzed for individual enzymes, including catalase, alkaline phosphatase and dehydrogenase, and the changes of microbial community structure. Results showed that heavy metals slightly inhibited the enzymatic activities in all the samples spiked with heavy metals. The extent of inhibition increased significantly with increasing level of heavy metals, and varied with the incubation periods. The soil bacterial community structure, as determined by polymerase chain reaction-denaturing gradient gel electrophoresis techniques, was different in the contaminated samples as compared to the control. The highest community change was observed in the samples amended with high level of Cd. Positive correlations were observed among the three enzymatic activities, but negative correlations were found between the amounts of the heavy metals and the enzymatic activities.

References (50)

  • WD Kong et al.

    The veterinary antibiotic oxytetracycline and Cu influence functional diversity of the soil microbial community [J]

    Environ Pollu

    (2006)
  • T Kunito et al.

    Copper and zinc fractions affecting microorganisms in long-term sludge-amended soils[J]

    Bioresource Technol

    (2001)
  • BJ Majer et al.

    Effects of heavy metals contamination on micronucleus induction in Traes-candia and on microbial enzymes activities: a comparison investigation[J]

    Mutation Res

    (2002)
  • JL Moreno et al.

    Toxicity of cadmium to soil microbial activity: effects of sewage sludge addition to soil on the ecological dose[J]

    Appl Soil Ecol

    (2002)
  • A Perez-de-Mora et al.

    Microbial community structure and function in a soil contaminated by heavy metals: effects of plant growth and different amendments[J]

    Soil Biol Biochem

    (2006)
  • RK Rattan et al.

    Long-term impact of irrigation with sewage effluents on heavy metal content in soils, crops and groundwater-a case study[J]

    Agr Ecosyst Environ

    (2005)
  • G Renella et al.

    Functional activity and microbial community structure in soils amended with bimetallic sludges[J]

    Soil Biol Biochem

    (2005)
  • G Shen et al.

    Interaction of polycyclic aromatic hydrocarbons and heavy metals on soil enzymes[J]

    Chemosphere

    (2005)
  • C Trasar-Cepeda et al.

    Limitation of soil enzymes as indicators of soil pollution[J]

    Soil Biol Biochem

    (2000)
  • M Akmal et al.

    Changes in enzymes activity, substrate utilization pattern and diversity of soil microbial communities under cadmium pollution[J]

    J Environ Sci

    (2005)
  • KM Anne et al.

    The effect of long-term mercury pollution on the soil microbial community[J]

    FEMS Microbiol Ecol

    (2001)
  • E Baath et al.

    Effect of metal-rich sludge amendments on the soil microbial community [J]

    Appl Environ Microbiol

    (1998)
  • ON Belyaeva et al.

    Barely yield and soil microbial and enzyme activities as affected by contamination of two soils with lead, zinc or copper[J]

    Biol Pert Soils

    (2005)
  • LE Casida et al.

    Soil dehydrogenase activity[J]

    Soil Sci

    (1964)
  • SG Claudia et al.

    Application of broad-range 16S rRNA PCR amplification and DGGE fingerprinting for detection of tick-infecting bacteria[J]

    J Microbiol Methods

    (2003)
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    Project supported by the Key Project of Chinese Academy of Sciences (No. KZCX3-SW-431, KZCX2-YW-408) and the National Natural Science Foundation of China (No. 40321101, 40571082).

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