In Vitro Antioxidant Activity Of Methanolic Extract Of Rhodiola Imbricata Edgew

doi:10.1016/S0975-3575(10)80084-7

Pharmacognosy Journal

Volume 2, Issue 7, March 2010, Pages 157-161

https://doi.org/10.1016/S0975-3575(10)80084-7 Get rights and content

Abstract

Rhodiola imbricata (Roseroot) is a perennial plant distributed in South Asia viz., Pakistan, Nepal, China and India. In India it is endemic to Trans Himalayan cold desert of Ladakh. It is a well known medicinal plant in the Amchi system of medicine (Tibetan system of medicine) being used in various ailments like anti-stress, radio-protective, anticancer, anti-inflammatory agent, adaptogen etc. The aim of this study was to assess the in vitro potential of methanol extract of Rhodiola imbricata roots. The DPPH activity of the extract (0.1–1.2 mg/ml) was increased in a dose dependent manner, which was found in the range of (39.55–70.76%) as compared to ascorbic acid (46.78–81.47%). The IC₅₀ values of methanol extract in DPPH radical, nitric oxide, hydroxyl radical were obtained to be 0.33, 0.47, 0.58 mg/ml, respectively. However, the IC₅₀ values for the standard ascorbic acid were noted to be 0.42, 0.43, 0.51 mg/ml, respectively. Measurement of total phenolic content of the methanol extract of R. imbricata was achieved using Folin-Ciocalteau reagent containing 185.7 mg/g of phenolic content, which was found significantly higher when compared to reference standard gallic acid. The results obtained in this study clearly indicate that R. imbricata has a significant potential to use as a natural anti-oxidant agent.

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Cited by (14)

Adaptogenic, immunomodulatory, and antioxidant activities of three Echeveria species (Crassulaceae)
2022, South African Journal of Botany
Citation Excerpt :
The antioxidant properties of different adaptogens have been reported (Ivanišová and Kacaniova, 2017). In this regard, the IC50 values (μg/mL) of the antioxidant activities of the Echeveria HE (Table 2) were lower than the reported for the adaptogens Rhodiola imbricata (DPPH, 330) (Kumar et al., 2010) and Withania somnifera (DPPH, 390; ABTS, 2140) (Dhanani et al., 2017), the activities were associated with the phenolics content for both plants. For Rhododendron adamsii (Ericaceae) leaves, extracts obtained with aqueous methanol mixtures showed better activity (DPPH, IC50 = 4.82–25.8 μg/mL) than the Echeveria HE (Table 2); the mixture (40% methanol) with the highest activity was enriched in phenolics and showed the best adaptogenic activity in the swimming to exhaustion test in mice (Olennikov et al., 2021).
Three Echeveria species (E. craigiana, EC; E. kimnachii, EK; and E. subrigida, ES) can potentially prevent/treat different diseases. This study aims to establish the following biological activities (evaluation assay) of their hydroalcoholic extracts (HE): adaptogenic (anoxia, forced-swim-endurance, and cold-swim-endurance in mice), immunomodulatory (splenocyte proliferation), antioxidant (ABTS, DPPH, and cellular-in-Saccharomyces cerevisiae), and toxicological (Artemia salina and acute in mice). EK and ES were the best adaptogens and immunomodulators (at 10 μg/mL: EK, 34%; ES, 20%). ES had the best antioxidant activities in vitro (DPPH, 1122.6 μmol TE/g, IC₅₀ = 200.7 μg/mL; ABTS, 2196.8 μmol TE/g, IC₅₀ = 165.8 μg/mL), and EK was the best in the Saccharomyces cerevisiae assay. The three HE of Echeveria were innocuous in the Artemia salina (LC₅₀ > 2000 mg/kg) and mice acute toxicity (LD₅₀ > 2 g/kg b.w.) assays. Thus, EK and ES showed the best potential for stress management.
A literature review of the studies concerning selected plant-derived adaptogens and their general function in body with a focus on animal studies
2022, Phytomedicine
Adaptogens are generally referred to the substances, mostly found in plants, which non-specifically increase resilience and chances of survival by activation of signaling pathways in affected cells.
This literature review was conducted to summarize the investigation, until March 2021, on selected adaptogenic plants and plant-derived substances.
Electronic databases were searched (up to March 2021) for in vitro and animal studies, as well as clinical trials. Moreover, all modes of action connected with the adaptogenic effects of plants and phytochemicals were collected.
The search of relevant studies was performed within electronic databases including Scopus, Science Direct, PubMed, and Cochrane library. The most important keywords were adaptogen, plant, phytochemical, and plant-derived.
The most investigated medicinal herbs for their adaptogenic activity are Eleutherococcus senticosus, Panax ginseng, Withania somnifera, Schisandra chinensis, and Rhodiola spp., salidroside, ginsenosides, andrographolide, methyl jasmonate, cucurbitacin R, dichotosin, and dichotosininare are phytochemicals that have shown a considerable adaptogenic activity. Phytochemicals that have been demonstrated adaptogenic properties mainly belong to flavonoids, terpenoids, and phenylpropanoid glycosides.
It is concluded that the main modes of action of the selected adaptogenic plants are stress modulatory, antioxidant, anti-fatigue, and physical endurance enhancement. Other properties were nootropic, immunomodulatory, cardiovascular, and radioprotective activities.
Therapeutic plants of ayurveda; a review on anticancer
2011, Pharmacognosy Journal
Cancer is actually a group of many related diseases that all have to do with cells. Cells are the very small units that make up all living things, including the human body. There are billions of cells in each person's body. Cancer happens when cells that are not normal grow and spread very fast. Normal body cells grow and divide and know to stop growing. Over time, they also die. Unlike these normal cells, cancer cells just continue to grow and divide out of control and don't die when they're supposed to. A number of synthetic anticancer drugs are available in practice, however their effectiveness does not hold true with the entire range of population suffering from this disorder. Moreover the side effects and the drug interactions are major restrictions in its clinical utility. On the other hand, herbal medicines are now attracting attention as potential sources of anticancer agents are widely used across the globe due to their wide applicability and therapeutic efficacy coupled with least side effects, which in turn has accelerated the scientific research regarding the anticancer activity. In this overview we have summarized the current research advancements on plants belongs to different families like Apocyanaceae, Taxaceae, Beriberidaceae, Solanaceae, Cupressaceae etc. having anticancer activities along with their other activities.
Antibacterial and antioxidant activities of ethanol extracts from trans Himalayan medicinal plants
2010, Pharmacognosy Journal
The antibacterial and antioxidant activity of the ethanol extracts from some trans Himalayan medicinal plants being used in ‘Amchi system of medicine’. These plants were assessed towards selected bacteria as well as in different antioxidant models. Extracts, at concentration between 8 and 250 µg/ml, showed a significant antibacterial effect expressed as minimum inhibition concentration (MIC) against both Gram-negative and Gram-positive bacteria. In particular, samples of Podophyllum hexandrum leaf, Verbascum thapsus stem against Bacillus subtilis and Salvia sclarea flower against Pseudomonas aeruginosa (MIC = 8 µg/ml) were highly effective. The antioxidant activity was determined by the 2,2- diphenyl -1 picryl hydrazyl (DPPH) method, Ephedra gerardiana leaf (13.30 ± 0.6 µg/ml) and Salvia sclarea flower (14.97 ± 2.9 µg/ml) were observed with maximum activity. Conclusion of the study supports the use of these plants in traditional medicine to treat various ailments like stomach complaints, wound infections and intestinal disorders etc.
Antioxidant and DNA damage preventive properties of Centella asiatica (L) Urb.
2010, Pharmacognosy Journal
Centella asiatica (L.) (Apiaceae) is commonly known as Mandukaparni. It is distributed in South America and all around Asia. It is a well known medicinal plant in Ayurveda system of medicine being used for various ailments like inflammation, diarrhea, asthma, tuberculosis, depression, memory loss and psoriasis. The aim of the present study was to assess the antioxidant potential in vitro of extracts of Centella asiatica in different solvents like hexane, chloroform, ethyl acetate, acetone, methanol and water. Highest polyphenols content was found in chloroform extract followed by methanol extract (9.04 µg/mg, 7.7 µg/mg gallic acid equivalents) and flavonoid content was found to be highest in water followed by chloroform extract (2.19 µg/g, 2.00 µg/g) respectively. The IC₅₀ value of the DPPH and hydroxyl radical scavenging activity of methanol extract showed 0.07 mg/ml and 500 µg/ml respectively. Reducing power assay results also followed in the same way. Methanol extract was comparatively effective in preventing more DNA damage. The results obtained in this study clearly indicate that C.asiatica has a significant potential as a natural anti-oxidant and DNA damage preventing agent.
Box-Behnken design optimization and kinetics of phenolics extraction from Etlingera elatior leaves
2023, AIP Conference Proceedings

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Pharmacognosy Journal

Abstract

References (23)

Indian medicinal herbs as sources of antioxidants

Food Res Int

Reduction and release of ferritin iron by plant phenolics

J. Inorg. Biochem

Flavonoids, a class of natural products of high pharmacological potency

Biocchem. Pharmacol

The nitric oxide scavenging properties of Gingo biloba extract EGb 761

Biochemical and Biophysical Research Communications

Antioxidant and free radical scavenging activities of polyphenol-enriched curry leaf extract (Murraya koenigii L.)

Food Chemistry

Free radicals in disease processes: A complication of cause and consequence

Free Radic. Res. Comm

Oxygen radicals in disease

Adv. Drug Reac. Bull

Review of the epidemiological evidence for the antioxidant hypotheses

Pub Health Nutr

Antioxidant potential of Ecklonia cava on reactive oxygen species scavenging metal chelating, reducing power and lipid peroxidation inhibition

Food SciTech Int

Screening of antioxidant activity of three Indian medicinal plants, traditionally used for the management of neurodegenerative diseases

J. Ethnopharmacol

Antioxidant flavonoids: structure, function and clinical usage

Alt. Med. Rev

Cited by (14)

Adaptogenic, immunomodulatory, and antioxidant activities of three Echeveria species (Crassulaceae)

A literature review of the studies concerning selected plant-derived adaptogens and their general function in body with a focus on animal studies

Therapeutic plants of ayurveda; a review on anticancer

Antibacterial and antioxidant activities of ethanol extracts from trans Himalayan medicinal plants

Antioxidant and DNA damage preventive properties of Centella asiatica (L) Urb.

Box-Behnken design optimization and kinetics of phenolics extraction from Etlingera elatior leaves