Influence of excluded volume upon macromolecular structure and associations in ‘crowded” media
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2022, Journal of Biological ChemistryCitation Excerpt :These data are consistent with a link between crowding, catalytic activity, and restructuring of the C-terminal tail around the PTP domain. A common effect of crowding agents is to promote the clustering of macromolecules because of the excluded volume effect (35, 42), which can directly influence their catalytic activity by limiting substrate access to the active site. To better understand the mechanism by which crowding acts on TC-PTP, we generated C- and N-terminal CFP and YFP fusions in all possible combinations for both TC45 and TC45E/A, including C-C and Y-Y species to be used as controls (Fig. 3D).
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