Gene expression pattern
MDM2 expression during mouse embryogenesis and the requirement of p53

https://doi.org/10.1016/S0925-4773(98)00074-4Get rights and content
Under an Elsevier user license
open archive

Abstract

We compared mouse embryonic expression of the MDM2 proto-oncogene, p21WAF1/CIP1 and their transcriptional regulator, p53. MDM2 expression is ubiquitous from 7.5 to 11.5 days post coitum (dpc) and more restricted from 12.5 dpc, with the highest levels in the testes and neural tube. From 14.5 to 18.5 dpc, the nasal respiratory epithelium expresses high levels of MDM2 RNA and protein and p21WAF1/CIP1 RNA, in both wild type and p53 null embryos. MDM2 expression during development is tissue-specific and, like p21WAF1/CIP1, is independent of p53. MDM2 may have a developmental role after 6.5 dpc, when MDM2 null mice die (Jones, S.N., Roe, A.E., Donehower, L.A., Bradley, A., 1995. Rescue of embryonic lethality in Mdm2-deficient mice by absence of p53. Nature 378, 206–208; Montes de Oca Luna, R., Wagner, D.S., Lozano, G., 1995. Rescue of early embryonic lethality in mdm2-deficient mice by deletion of p53. Nature 378, 203–206).

Keywords

MDM2
p53
p21
WAF1
CIP1
p53 Null
Testes
Neural tube
Bronchi
Kidney cortex
Lung
Oesophagus
Sinus
Teeth
Thymus
Eustachian tube
Lens
Nasopharynx
Retina
Trachea
p53-Independent expression
MDM2 protein
In situ hybridisation
Western blot
Respiratory epithelium
Oncogene
Tumour suppressor
Apoptosis
pRb

Cited by (0)

1

Present address: Laboratoire de Physiopathologie Retinienne, Université Louis Pasteur, CHRU, 67091 Strasbourg Cedex, France.

2

Present address: Laboratoire de Biologie Cellulaire, Faculte de Medecine, Universite de Bordeaux II, 33076 Bordeaux Cedex, France.