Elsevier

Sensors and Actuators B: Chemical

Volume 38, Issues 1–3, January–February 1997, Pages 110-115
Sensors and Actuators B: Chemical

Oxygen flux fluorescence lifetime imaging

https://doi.org/10.1016/S0925-4005(97)80179-7Get rights and content

Abstract

We describe a device capable of imaging distributions of surface PO2 as well as oxygen flux (e.g., into the human skin). Oxygen is monitored by a phase-angle-sensitive imaging technique using the effect of luminescence lifetime quenching of ruthenium complexes incorporated into a polymer layer system. The dyes are excited by modulated radiation of blue light-emitting diodes. The phase-shifted luminescence is detected by an intensified CCD camera. Several modulation techniques have been comparatively investigated. The obtained images correspond pixel-by-pixel to the local luminescence lifetimes, which are a direct measure for the distribution of surface PO2, or alternatively for the oxygen flux through a permeable diffusion barrier. For an illuminated sensor area of 10 cm2 and a spatial resolution of 1 mm at the sensor layer, the oxygen resolution is better than ΔPO2 = 0.3 torr in the absence of oxygen, and better than ΔPO2 = 4 torr at PO2= 160 torr.

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  • Cited by (0)

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    Present address: Max-Planck-Institut für marine Mikrobiologie, Celsiusstraβe 1, D-28359 Bremen, Germany.

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