Identification of viruses with bi- and trisegmented double-stranded RNA genome in faeces of children with gastroenteritis

doi:10.1016/S0923-2516(06)80032-4

Research in Virology

Volume 144, 1993, Pages 219-224

Research in Virology

https://doi.org/10.1016/S0923-2516(06)80032-4 Get rights and content

Summary

Polyacrylamide gel electrophoresis of nucleic acid extracted from faecal samples of diarrhoeic children revealed the presence of group A rotavirus in 50 (23.4 %) samples and group C rotavirus in 1 (0.5 %) sample out of 214 tested. One other sample showed the presence of three bands (with apparent length of 2.92, 2.37 and 1.32 kbp) which by enzymatic digestion analysis, were shown to consist of double-stranded RNA (dsRNA). The sample was shown by electron microscopy to contain virus particles with a diameter of 32–34 nm. On the basis of morphology and genomic characteristics, this virus closely resembles a virus hitherto described only in chickens by Leite et al. in 1990 and tentatively named “picotrirnavirus”. From the same group of 214, one sample containing a “picobirnavirus” was also identified. Thus, small icosahedral viruses with either a bi-or trisegmented dsRNA genome appear to infect humans. However, their pathogenic potential remains to be established.

L'électrophorèse en gel de polyacrylamide des acides nucléiques extraits de 214 échantillons de selles diarrhéiques d'enfants, a révélé la présence de rotavirus du groupe A dans 50 échantillons (23,4 %), de rotavirus du groupe C dans 1 échantillon (0,5 %) et de trois bandes (d'une longueur apparente de 2,92, 2,37 et 1,32 kbp), dans un autre échantillon.

La digestion enzymatique a montré que le matériel est formé d'ARN double-brin. L'analyse au microscope électronique a mis en évidence la présence de particules virales d'un diamètre de 32–34 nm. Etant donné la morphologie et les caractéristiques du génome, ce virus ressemble fortement à un virus décrit, jusqu'à présent, seulement chez les poulets (par Leite et coll. en 1990) et dont l'appellation proposée est «picotrirnavirus. Un échantillon contenant un «picobirnavirus a été identifié dans le même groupe. Ainsi, ces virus icosahédriques au génome caractérisé par un ARN double-brin bi- ou trisegmenté semblent infecter les humains, bien que leur potentiel pathogène n'ait pas encore été établi.

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Citation Excerpt :
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PBVs were initially discovered in humans by the finding of the two segments of dsRNA by PAGE, extracted from a small number of fecal specimens during investigation of gastroenteritis in children in 1988.2 Since then, viruses with similar characteristics have been detected in fecal samples from domestic and wild animal species3–6 as well as in stool samples from humans with and without diarrhea.7–17 At present, PBV excretion is not definitely linked to any pathological situation.
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Identification of viruses with bi- and trisegmented double-stranded RNA genome in faeces of children with gastroenteritisIdentification de virus ARN double-brin bi- et trisegmenté dans les selles d'enfants atteints de gastroentérite

Summary

Res. Vet. Sci.

Virus Res.

Lancet

Viral gastroenteritis

New Engl. J. Med.

Bisegmented ds viruses (Picobirnaviruses) in man: preliminary studies, in VIIIth International Congress of Virology Abstracts

Novel agents of viral enteritis in humans

J. Infect. Dis.

Cleavage of structural proteins during the assembly of the head of bacteriophage T4

Nature (Lond.)