Original articleSpecific detection of Campylobacter concisus by PCR amplification of 23S rDNA areas
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Cited by (33)
The ribosomal RNA operon (rrn) of Campylobacter concisus supports molecular typing to genomospecies level
2017, Gene ReportsCitation Excerpt :Later C. concisus has been isolated from intestinal biopsies and faecal samples of children with Crohn's disease (CD) (Zhang et al., 2009; Kaakoush et al., 2011). C. concisus is a heterogeneous species, consisting of two or more genomospecies which are phenotypically indistinguishable but genetically distinct taxa (Vandamme et al., 1989; Bastyns et al., 1995; On and Harrington, 2000; Aabenhus et al., 2002a; Istivan et al., 2004; Aabenhus et al., 2005a; Engberg et al., 2005). Previously, phenotypically confirmed faecal C. concisus isolates were shown to have only 42 to 50% DNA-DNA hybridization values with reference strains of oral origin (Vandamme et al., 1989).
Antibiotic susceptibility pattern and genotyping of campylobacter species isolated from children suffering from gastroenteritis
2014, Indian Journal of Medical MicrobiologyOccurrence and characteristics of fastidious Campylobacteraceae species in porcine samples
2013, International Journal of Food MicrobiologyCitation Excerpt :Control culture collection strains (Table 1) were included in the PCR assays used to identify isolates to genus or selected species level. Isolates identified as Campylobacter spp. were then speciated as C. coli, C. jejuni, C. upsaliensis, and C. lari using primers described by Klena et al., 2004, as Campylobacter helveticus, C. fetus, C. curvus, C. sputorum and C. mucosalis using PCR as described by Lynch et al. (2010 and 2011) or as C. concisus using PCR developed by Bastyns et al. (1995a). Isolates identified as Arcobacter spp. were speciated (A. butzleri, A. cryaerophilus and Arcobacter skirrowii) using primers designed by Houf et al. (2000).
Occurrence of fastidious Campylobacter spp. in fresh meat and poultry using an adapted cultural protocol
2011, International Journal of Food MicrobiologyCitation Excerpt :Amplifications were carried out in 50 μl reactions composed of 2.5 U Taq PCR polymerase, 1 × PCR buffer, 200 μm dNTPs (Qiagen), 10 pmol/μl each forward primer, 30 pmol/μl reverse primer and 3 μl template DNA (Klena et al., 2004). Primers targeting a 306 bp region of 23 s rDNA were used to differentiate between C. concisus and the biochemically similar species C. mucosalis (Bastyns et al., 1995). Amplifications were carried out in 50 μl reactions composed of 2.5 U Taq PCR polymerase, 1 × PCR buffer, 200 μm dNTPs (Qiagen), 5 pmol/μl each Con1 and Con2 primers, 10 pmol/μl Muc1 primer, 2.4 μl 25 mM MgCl2 and 3 μl template DNA.
A method for the growth and recovery of 17 species of Campylobacter and its subsequent application to inoculated beef
2010, Journal of Microbiological MethodsCampylobacter concisus: An evaluation of certain phenotypic and genotypic characteristics
2005, Clinical Microbiology and InfectionCitation Excerpt :PCR amplification of 23S rDNA This was performed by the method of Bastyns et al. [17] with modifications [11]. The method was modified by using the two reverse primers (CON1 and CON2) independently, rather than as a mixture, and was used to group the isolates.