Isolation and Characterization of Burkholderia norimbergensis sp. nov., a Mildly Alkaliphilic Sulfur Oxidizer

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Summary

A new bacterium (“DSM11628”) capable of heterotrophic sulfur oxidation under slightly alkaline conditions (pH 8.4) was isolated from an oxic water layer above a sulfide containing sediment. A comparison of 16S rDNA sequences reveals its association with the genus Burkholderia (formerly Pseudomonas rRNA group II). The isolate is a strict aerobe and oxidizes reduced sulfur compounds, utilizes a number of mono- and disaccharides as well as Krebs cycle intermediates as carbon and energy sources, but fixes neither CO2 nor N2. Based on phenotypic and phylogenetic considerations, a new species, Burkholderia norimbergensis is proposed.

References (20)

  • M. Mandel et al.

    Use of ultraviolet absorbance temperature profile for determining the guanine plus cytosine content of DNA

    Methods Enzymol.

    (1968)
  • J. De Ley

    Reexamination of the association between melting point, buoyant density, and chemical base composition of deoxyribonucleic acid

    J. Bacteriol.

    (1970)
  • M. Gillis et al.

    Polyphasic taxonomy in the genus Burkholderia leading to an amended description of the genus and proposition of Burkholderia vietnamensis sp. nov. for N2 fixing isolates from rice fields in Vietnam

    Int. H. Syst. Bacteriol.

    (1995)
  • K. Kersters et al.

    Recent changes in the classification of the pseudomonads: a overview

    System. Appl. Microbiol.

    (1966)
  • J.G. Kuenen et al.

    The genera Thiobacillus, Thiomicrospira and Thiosphaera

  • X. Li et al.

    Phylogenetic studies on the RNA group II pseudomonads based on 16S-rRNA gene sequences

    J. Appl. Bacteriol.

    (1993)
  • O. Lysenko

    Pseudomonas — an attempt at a general classification

    J. Gen. Microbiol.

    (1961)
  • B.L. Maidak et al.

    The ribosomal database project

    Nucleic Acid Res.

    (1994)
  • R.V. Masterson et al.

    Conservation of symbiotic nitrogen fixation gene sequences in Rhizobium japonicum and Bradyrhizobium japonicum

    J. Bacteriol.

    (1985)
  • L.T. Miller

    A single derivatization method for bacterial fatty acid methyl esters including hydroxy acids

    J. Clin. Microbiol.

    (1982)
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