Morphological and biochemical characterization and emended descriptions of thermophilic actinomycetes species

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Summary

Thermophilic species of nine genera of Actinomycetales were characterized by morphology, biochemical markers, physiology and phage-typing. The results of this study led to a key for the identification of the genera Actinomadura, Faenia, Microbispora, Micropolyspora, Pseudono cardia, Saccharomonospora, Saccharopolyspora and Thermomonospora. On the basis of our biochemical data, the following species, most of them not included in the Approved Lists of Bacterial Names, are described: Actinomadura angiospora comb. nov. (formerly Micropolyspora angiospora), Actinomadura rubrobrunea nom. rev., comb. nov. (containing the two former species ‘Excellospora rubrobrunea” and ‘Excellospora viridinigra’), Saccharomonospora caesia nom. rev., comb. nov. (formerly ‘Micropolyspora caesia’), Saccharomonospora internatus comb. nov. (formerly Micropolyspora internatus) and Thermopolyspora flexuosa nom. rev., comb. nov. (formerly ‘Actinomadura flexuosa’). In the case of Microbispora, Saccharomonospora and Thermomonospora further studies are needed for species identification.

References (50)

  • M.D. Collins et al.

    Menaquinone composition of members of the genus Thermoactinomyces

    FEMS Microbiol. Lett.

    (1982)
  • N.S. Agre et al.

    New genus of actinomycetes: Excellospora gen. nov.

    Microbiology (engl. transl. of Microbiologiya)

    (1975)
  • N.S. Agre et al.

    A new species of the genus Micropolyspora — Mip. internatus sp. nov.

    Microbiology (Engl. transl. of Microbiologiya)

    (1974)
  • H. Claus

    Untersuchungen über die Tyrosinase bei Melanin-bildenden Streptomyceten

    Ph. D. Thesis, TH Darmstadt

    (1983)
  • M.D. Collins et al.

    Distribution of menaquinones in actinomycetes and corynebacteria

    J. Gen. Microbiol.

    (1977)
  • T Cross

    Microbispora Nonomura and Ohara

  • T. Cross

    The monosporic actinomycetes

  • T. Cross et al.

    Taxonomy and classification of the actinomycetes

  • T. Cross et al.

    A new genus of the Actinomycetales: Microellobosporia gen. nov.

    J. Gen. Microbiol.

    (1963)
  • T. Cross et al.

    The thermophilic actinomycetes in moldy hay: Micropolyspora faeni sp. nov.

    J. Gen. Microbiol.

    (1968)
  • G. Pennington et al.

    Cellulase biosynthesis in a catabolite repression-resistant mutant of Thermomonospora curvata

    App. Environm. Microbiol.

    (1984)
  • A. Fischer et al.

    Moleculargenetic and chemotaxonomic studies on Actinomadura and Nocardiopsis

    J. Gen. Microbiol.

    (1983)
  • N.N. Gerber et al.

    Phenazines and phenoxazinones from Waksmania aerata sp. nov. and Pseudomonas iodina

    Biochemistry

    (1964)
  • A. Henssen

    Beiträge zur Morphologie und Systematik der ther-mophilen Actinomyceten

    Arch. Mikrobiol.

    (1957)
  • L.V. Kalakoutskii

    A new species of the genus Micropolyspora—Mip. caesia sp. nov.

    Microbiology (engl. transl. of Microbiologiya)

    (1964)
  • F. Korn et al.

    A study of 20 ac-tinophages: Morphology, serological relationship and host range

  • N.A. Krasil'nikov et al.

    On two new species of Thermopolyspora

    Hind. Antibiot. Bull.

    (1964)
  • N.A. Krasil'nikov et al.

    The brown group of Actinobifida, Actinobifida chromogena n. sp.

    Microbiology (engl. transl. of Microbiologiya)

    (1965)
  • N.A. Krasil'nikov et al.

    New thermophilic species of the genus Micropolyspora

    Microbiology (engl. transl. of Microbiologiya)

    (1968)
  • R.M. Kroppenstedt

    Separation of bacterial menaquinones by HPLC using reverse phase (RP 18) and a silver loaded ion exchanger

    J. Liquid Chromatogr.

    (1982)
  • R.M. Kroppenstedt

    Fatty acid and menaquinone analysis of actinomycetes and related organisms

  • V.P. Kurup

    Characterization of some members of the genus Thermomonospora

    Curr. Microbiol.

    (1979)
  • V.P. Kurup

    Taxonomic study of some members of Micropolyspora and Saccharomonospora

    Microbiologica

    (1981)
  • V.P. Kurup et al.

    Transfer of Micropolyspora rectivirgula (Krassilnikov and Agre, 1964) Lechevalier, Lechevalier and Becker 1966 to Faenia gen. nov.

    Int. J. system. Bact.

    (1983)
  • V.P. Kurup et al.

    A scheme for the identification of thermophilic actinomycetes associated with hypersensitivity pneumonitis

    J. Clin. Microbiol.

    (1975)
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