Announcement of population dataTunisian population data on 10 Y-chromosomal loci
Section snippets
Population
The examined population is constituted by 100 unrelated Arabic-speaking individuals coming from the three different regions of Tunisia: North, Centre and South.
Extraction
Blood samples on FTA™ paper were punched and extracted with purification reagent FTA [1].
PCR
According to manufacturer’s instructions (Reliagene Technologies Inc., New Orleans, LA).
Typing
ABI310 (Applied Biosystems) and reference sequencer ladders of the Reliagen Y-Plex™6 and Y-Plex™5 kits.
Quality control
Y-Plex™6 Reliagene validation, proficiency testing of the GEDNAP, French-speaking group of ISFG.
Results
See Table 1, Table 2.
Analysis of data
Allele frequencies were calculated by direct counting. Gene and haplotype diversity were calculated as proposed by Nei [2].
Remarks
Seventy-one different haplotypes were identified in a Tunisian population of 100 unrelated males coming from the three regions of the country (North, Centre and South). The most common haplotypes were the numbers 9, 13 and 22 which were encountered each in six individuals. Some rare alleles have been found: twice the allele 11 at the locus DYS19, alleles 13.2 (four times) and 21 (twice) at DYS385 I/II, allele 27 (twice) at DYS389II, allele 21 (twice) at DYS390, allele 13 (once) at DYS391,
Acknowledgements
The authors wish to thank Mrs A. Grini for technical assistance.
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