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Tunisian population data on 10 Y-chromosomal loci

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Abstract

Alleles and haplotypes frequencies for 10 Y-chromosome STR loci (DYS19, DYS385 I/II, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS438 and DYS439), included in the Y-Plex™6 and Y-Plex™5 kits were determined for a Tunisian population sample of 100 male individuals.

Section snippets

Population

The examined population is constituted by 100 unrelated Arabic-speaking individuals coming from the three different regions of Tunisia: North, Centre and South.

Extraction

Blood samples on FTA™ paper were punched and extracted with purification reagent FTA [1].

PCR

According to manufacturer’s instructions (Reliagene Technologies Inc., New Orleans, LA).

Typing

ABI310 (Applied Biosystems) and reference sequencer ladders of the Reliagen Y-Plex™6 and Y-Plex™5 kits.

Quality control

Y-Plex™6 Reliagene validation, proficiency testing of the GEDNAP, French-speaking group of ISFG.

Results

See Table 1, Table 2.

Analysis of data

Allele frequencies were calculated by direct counting. Gene and haplotype diversity were calculated as proposed by Nei [2].

Remarks

Seventy-one different haplotypes were identified in a Tunisian population of 100 unrelated males coming from the three regions of the country (North, Centre and South). The most common haplotypes were the numbers 9, 13 and 22 which were encountered each in six individuals. Some rare alleles have been found: twice the allele 11 at the locus DYS19, alleles 13.2 (four times) and 21 (twice) at DYS385 I/II, allele 27 (twice) at DYS389II, allele 21 (twice) at DYS390, allele 13 (once) at DYS391,

Acknowledgements

The authors wish to thank Mrs A. Grini for technical assistance.

References (6)

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